Waters XSelect CSH XP 2.5 µm Columns - Manual

XSelect CSH XP 2.5 µm Columns 1
[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
II. GETTING STARTED
a. Column Connection
b. Column Installation
c. Minimizing Band Spread Volume
d. Measuring Band Spread Volume
e. Measuring System Dwell Volume
f. Column Equilibration
g. eCord Installation
h. Initial Column Efficiency Determination
i. VanGuard Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
IV. COLUMN CLEANING, REGENERATION AND STORAGE
a. Cleaning and Regeneration
b. Storage after Reversed-Phase Use
V. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing
d. Column Use
VI. ADDITIONAL INFORMATION
a. Tips for Maximizing XSelect CSH XP 2.5 µm Column Lifetime
b. Troubleshooting Questions
c. Recommended Flow Rates and Anticipated Backpressures for
Reversed-Phase XSelect CSH XP 2.5 µm Columns
XSELECT CSH XP 2.5 µm COLUMNS
I. INT RODUCTION
Thank you for choosing a Waters XSelect ™ Charged Surface Hybrid
[CSH] eXtended Performance [XP] 2.5 µm Column. The manufacture
of XSelect CSH XP 2.5 µm Columns begins with ultrapure reagents
and are manufactured in a cGMP, ISO 9001 certified facility to
control the chemical composition and purity of the final product.
Well-controlled manufacturing processes result in industry-leading
batch-to-batch reproducibility. Every column is individually tested.
A Performance Chromatogram and Certificate of Batch Analysis are
provided on the eCord
™ Intelligent Chip.
XSelect CSH XP 2.5 µm Columns are based on the same base particle
technology and bonded-phase chemistry as 1.7 µm ACQUITY UPLC
®
CSH Columns as well as XSelect CSH 3.5 and 5 µm HPLC Columns,
thus enabling seamless transferability between HPLC, UHPLC and
UPLC
® platforms.
XSelect CSH XP 2.5 µm Columns will exhibit maximum chromatographic
performance when used on a member of the ACQUITY UPLC
System family.