Waters Oligonucleotide Separation Technology XBridge OST C18 Columns - Manual (Page 7)

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June 2012 720001463 SC-IH
c. Impact of Bandspreading Volume on 2.1 mm i.d.
Column Performance
Use of a 2.1 mm i.d. XBridge OST C
18
column may require modification
to the HPLC system tubing in order to eliminate excessive system
bandspreading volume. Without proper system modification,
excessive system band-spreading volume will cause peak broadening
and will have a detrimental impact on peak resolution. For example,
an HPLC System with 70 μL bandspreading might yield 10,000
plates while the same column tested on a LC System with 130 μL
bandspreading would yield only 8,000 plates.
Note: Flow splitters after the column will introduce additional bandspreading.
System optimization, especially in a system that contains a flow splitter,
can have dramatic effects on sensitivity and resolution. Optimization includes
using correct ferrule depths and minimizing tubing inner diameters and
lengths. An example is given in Figure 9 where system optimization
resulted in a doubling of component sensitivity and resolution in an LC/
MS/MS system.
Figure 9: Non-Optimized vs. Optimized LC/MS/MS System
Table 3: XBridge OST C
18
Ordering Information
Description Particle Size Pore Size Dimension Part No.
XBridge OST C
18
2.5 μm 135Å 2.1 x 50 mm 186003952
XBridge OST C
18
2.5 μm 135Å 4.6 x 50 mm 186003953
XBridge OST C
18
2.5 μm 135Å 10.0 x 50 mm 186003954
Custom
XBridge OST C
18
-- -- -- 186003955
7.0 7.5
Non-optimized LC/MS/MS System Optimized System
8.0 7.0 7.5 8.0 min min