Waters 30 mm ID Preparative Guard Cartridges and Guard Holders - Manual (Page 4)

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[ CARE AND USE MANUAL ]
30 mm ID Preparative Guard Cartridges and Guard Holders
IX. GUARD CARTIDGE STORAGE
a. HPLC Guard Cartridges
To maintain the integrity of the bonded phase as well as the guard cartridge hardware and frits, flush
the guard cartridge with at least eight column volumes of 100% acetonitrile after every use. Never
store the guard cartridge in methanol or aqueous methanol. If the mobile phase contains a buffer salt,
first flush the guard cartridge with at least ten column volumes of 18 MegaOhm water followed by at
least eight column volumes of 100% acetonitrile. Please refer to Section II for the column volume.
b. SFC Guard Cartridges
Purging the SFC prep guard cartridge in pure CO
2
is recommended prior to storage. This will eliminate
or minimize retention changes of the guard cartridge during storage. Storing the guard cartridge in
methanol is not recommended.
VIII. TROUBLESHOOTING
Troubleshooting Tips
Symptom Cause Corrective Action
Solvent leak at seam
of cartridge weep hole
Cartridge not seated in end fitting Disassemble, properly seat cartridge
Particles on the face of the
O-ring or on metal seat
Wipe clean with a cotton tip applicator
Worn O-ring Replace O-ring
Broad peaks, loss
of peak resolution
Packing material fouled or voided Replace with new guard cartridge
Guard cartridge packing material
different from prep column packing
Replace with matching guard cartridge
High backpressure
Filters in guard cartridge
plugged or fouled
Replace with new guard cartridge
Guard cartridge chemically fouled Replace with new guard cartridge