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Care and use manual – Waters 30 mm ID Preparative Guard Cartridges and Guard Holders User Manual

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[ CARE AND USE MANUAL ]

30 mm ID Preparative Guard Cartridges and Guard Holders

VI. WHEN TO REPLACE THE GUARD CARTRIDGE

Replace guard cartridges before contaminants affect your
separation. For best results, replace the guard cartridge when:

1. The quality of your separation deteriorates.

2. The initial backpressure increases significantly.

(more than 20 percent).

3. Contaminant peaks appear in the chromatogram.

Weep Hole

Figure 2. Weep Hole on the Side of the Prep Guard Holder.

VII. REPLACING THE O-RINGS

The O-rings should be replaced if leaks from the cartridge
holder are observed. You will need replacement part number
186007012 (O-rings - 30 mm ID Prep Guard Holder, 2/pk),
a pointed device (such as a needle or dental pick), and a clear
workspace where small parts are easily retrieved if dropped.

To replace the O-rings:

1. Remove the cartridge holder from the system.

2. Unscrew the holder cap from the holder body.

3. Remove the guard cartridge.

4. Using a sharp needle, pry the O-rings from their grooves in

the holder cap and body. Be careful not to scratch the groove,
this may lead to leaks. It may be helpful to insert the needle
partway into the O-ring in order to remove it.

5. Examine the grooves and new O-rings. Remove any particulate

matter with a cotton tip applicator.

6. Install the new O-rings into the grooves. To ensure O-rings are

installed properly in the groove, insert guard cartridge into
holder body or holder cap and rotate 1/4 turn, applying light
hand pressure against the cartridge.

7. Re-assemble holder with cartridge and tighten according to

directions above.

Figure 3. Prep Guard Cartridge O-ring, and Needle/Dental Pick.

Needle or Dental Pick

O-ring