Leica Biosystems ASP200 S - Manual (Page 72)

72 Instructions for Use V 1.4, RevE – 03/2013
6. Troubleshooting
6.4 Typical fill or drain problems
Fill and drain problems may be due to several causes:
1. There is insufficient reagent
• Verify that the fill level of the reagent containers and the paraffin
reservoirs is sufficient.
2. Insufficient pressure or vacuum
The instrument is unable to create sufficient vacuum (for a fill) or pres-
sure (for a drain) in the retort.
• Verify that the reagent container in question is correctly inserted in
the reagent cabinet.
• Verify that all reagent containers are fully pushed home into their
sockets.
• Check whether there is some debris under the retort lid seal caus-
ing a leak.
3. There is a blockage in the air or reagent lines
Blockages in the reagent lines are typically caused by paraffin or tis-
sue debris. If there is reagent in the retort that cannot be drained, try to
remove the blockage as follows:
• Heat the retort to the maximum possible temperature (consistent
with the reagent currently in the retort, if any). Leave the retort at
this temperature for at least 15 minutes.
• While the retort is at maximum temperature, attempt to fill or drain.
• If there is no reagent in the retort:
Run the extended retort cleaning program. Use the maximum set-
tings for number of clean cycles and temperature (consistent with
the type of reagents/solvents used in the clean program).
If you cannot eliminate the problem by carrying out the above list-
ed steps, call Leica Technical Service before attempting to run any
further programs.