Eppendorf AF2200 Plate Reader - Manual (Page 63)

Technical data
Eppendorf ® PlateReader AF2200
English (EN)
63
9.6.1 Definition of the detection limit
The detection limit is the fluorophore concentration at which the signal that is corrected by the background
is equal to three times the standard deviation of the background noise.
When 1 flash per well is selected, the plate compartment does not stop at the measuring position. The
detection limit can be improved with more flashes per well, but the total measurement time will be longer.
9.6.2 Upper fluorescein (fluorescence intensity)
9.6.3 Lower fluorescein (fluorescence intensity)
Plate type (number of wells) 96
Dispensed volume [μL] 200
Flashes (measurements) per well 25
Fluorescin detection limit [pM] < 20 pM
Uniformity with 25 nM fluorescein <3%
Reproducibility with 25 nM fluorescein <2%
Plate type (number of wells) 96
Dispensed volume [μL] 200
Flashes (measurements) per well 25
Fluorescin detection limit [pM] < 100 pM
Uniformity with 25 nM fluorescein <3%
Reproducibility with 25 nM fluorescein <2%