Eppendorf AF2200 Plate Reader - Manual (Page 59)

Maintenance
Eppendorf ® PlateReader AF2200
English (EN)
59
Pipette 200 μL of 25 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells
according to the plate layout below.
Material/reagents
• 25 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)
• 0.01 M NaOH (=blank solution) (NaOH pellets)
• 96-well plate
• 200 μL pipette with tips
Calculating the precision from one measurement to the next
The result of the formula specifies the precision from one measurement to the next in % CV (coefficient of
variation).
X 25 nM fluorescein
blank 0.01 M NaOH
Mean value
Well A1
Mean RFU value of well A1 over 20 kinetic cycles
Std. deviation
Well A1
Standard deviation of RFU values from well A1 over
the 20 cycles
wellA1
wellA1
mean
100 * stdev
(CV%) Precision =