Eppendorf AF2200 Plate Reader - Manual (Page 60)

Maintenance
Eppendorf
® PlateReader AF2200
English (EN)
60
8.6.4 Absorbance test instructions - baseline stability
Conduct the following measurements to determine the baseline stability using the available filters.
Calculating the baseline stability
Calculate the standard deviation for each wavelength over 20 cycles.
Checking the accuracy of the absorbance
Parameter Setting
Measuring mode Absorbance
Wavelength 340 nm
405 nm
492 nm
590 nm
620 nm
700 nm
Number of flashes 25
Setting time 0 (μs)
Part of plate A1
Kinetic cycles 20, minimum time interval
• No plate is required for the measurement – the plate compartment must be empty for this
measurement.
• No materials or reagents are needed for this test.
Contact Eppendorf Service to have the accuracy of the absorbance checked.