2 filter slides and filter orientation, 1 filter slides, 2 inserting optical filters – Eppendorf AF2200 Plate Reader User Manual

Page 30: Filter slides and filter orientation 5.2.1, Filter slides, Inserting optical filters, Em ex

Inserting the filter slide
Eppendorf

PlateReader AF2200

English (EN)

5.2

Filter slides and filter orientation

5.2.1

Filter slides

The filter slides consist of an excitation section and an emission section. The filter slides allow the user to
work with four independent excitation/emission filter pairs which can be defined on positions 1 to 4. The
information of the inserted filters is saved on the integrated microchip.

Abb. 5-1: Filter slides (the arrows indicate the direction of the light)

Fig. 5-1:

Filter slides (the arrows indicate the direction of the light)

5.2.2

Inserting optical filters

There are two types of filters:

• Filters that are marked with an arrow

• Filters that are not marked

Both filter types must be correctly inserted into the filter slides to ensure that the light enters the filter from
the correct side.

For the predefined Eppendorf filter slides, the filters are permanently installed. They cannot
be removed or exchanged.

EX Excitation side (light entry)

EM Emission side (light outlet)

Abb. 5-2: Filter with marking (arrow indicates the direction of the light)

Fig. 5-2:

Filter with marking (arrow indicates the
direction of the light)

1. Insert the filter with the arrow marking into the

filter slide. On the excitation side of the filter
slide, the arrow points from the outside to the
inside and, on the emission side, from the inside
to the outside.