Eppendorf AF2200 Plate Reader - Manual (Page 51)

Maintenance
Eppendorf ® PlateReader AF2200
English (EN)
51
8.6.2 Test instructions, upper fluorescence
The following tests can be conducted on the device to check the device specifications for this measuring
procedure:
• Sensitivity
• Precision within a plate
• Precision from one measurement to the next
Sensitivity
Conduct the measurement with the following settings to determine the detection limit for fluorescein.
Pipette 200 μL of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells
according to the plate layout below.
Parameter Setting
Measuring mode Upper fluorescence
Wavelength, excitation 485 (20 nm)
Wavelength, emission 535 (25 nm)
Number of flashes 25
Integration time 40 (μs)
Setting time 0 (μs)
Signal gain Optimal
Plate type GRE96fb
X 1 nM fluorescein
blank 0.01 M NaOH