Eppendorf AF2200 Plate Reader - Manual (Page 58)

Maintenance
Eppendorf
® PlateReader AF2200
English (EN)
58
Calculating the precision across the plate
The results of the formula specifies the precision across the plate in % CV (coefficient of variation).
Precision from one measurement to the next
Carry out the following measurement to determine the precision and reproducibility.
Mean value
F
Mean RFU value of the wells filled with fluorophore
Std. deviation
F
Standard deviation of the RFU values of the wells
filled with fluorophore
Parameter Setting
Measuring mode Lower fluorescence
Wavelength, excitation 485 (20 nm)
Wavelength, emission 535 (25 nm)
Number of flashes 25
Integration time 40 (μs)
Setting time 0 (μs)
Signal gain Optimal
Plate type GRE96fb
Part of plate A1
Kinetic 20 cycles
Time interval Minimum
F
F
mean
100 * stdev
(%) Uniformity =