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6 absorbance measurement system, Absorbance measurement system – Eppendorf AF2200 Plate Reader User Manual

Page 19

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Product description

Eppendorf

®

PlateReader AF2200

English (EN)

19

2.6

Absorbance measurement system

A light path similar to the one used for fluorescence excitation is implemented for absorbance
measurements. The absorbance measurement module is located below the plate compartment. It measures
the light that is transmitted through the sample. A reference measurement, during which the plate
compartment is positioned outside of the light beam, is carried out before the microplate is measured.

Abb. 2-4: Absorbance measurement system

Fig. 2-4:

Absorbance measurement system

1

Light source

2

Absorbance optics

3

Absorbance detector

MTP

flash

lamp

condensor

reference
diode

beam

splitter

absorbance

absorbance

photo diode

1

2

2

3

filter