Eppendorf AF2200 Plate Reader - Manual (Page 52)

Maintenance
Eppendorf
® PlateReader AF2200
English (EN)
52
Material/reagents
• 1 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)
• 0.01 M NaOH (=blank solution) (NaOH pellets)
• 96-well plate
• 200 μL pipette with tips
Calculating the detection limit (sensitivity)
The result of the detection limit formula specifies the sensitivity in pM units.
Precision within a plate
Carry out the following measurement to determine the precision and reproducibility within a plate.
Concentration
F
Concentration of fluorophore in pM units
Mean value
F
Mean RFU value of the wells filled with fluorophore
Mean value
B
Mean RFU value of the wells filled with the blank
solution
Std. deviation
B
Standard deviation of the RFU values of the wells
filled with the blank solution
Parameter Setting
Measuring mode Upper fluorescence
Wavelength, excitation 485 (20 nm)
Wavelength, emission 535 (25 nm)
Number of flashes 25
Integration time 40 (μs)
Setting time 0 (μs)
Signal gain Optimal
Plate type GRE96fb
( )
B
B F
F Stdev * 3 *
mean mean
ion Concentrat
imit DetectionL
−
=