Eppendorf AF2200 Plate Reader - Manual (Page 18)

Product description
Eppendorf
® PlateReader AF2200
English (EN)
18
The lens system
The lens system bundles the maximum amount of fluorescent light from a well and focuses it onto the
detection system through the exit aperture.
The lenses are made of quartz glass. This material is extremely UV-transparent and virtually
non-autofluorescent.
2.5.3 Lower fluorescence optics
Flash light enters the optical system focused through an aperture. The opening functions as a color-specific
light source. The lower excitation fiber guides the light onto the lower measuring head, which consists of
an elliptical mirror. The mirror focuses the light into the well through the base of the microplate.
The emitted light will be focused onto the lower emission fiber which guides the light into the fluorescence
detection system via a mirror and through the emission filter.
2.5.4 Fluorescence detection
Emission filter
Emission filters are used to differentiate between non-specific fluorescence signals and sample-specific
fluorescence signals. The filters are mounted in removable slides. The spot diameter of the light beam on
the filters is approx. 2 mm for the upper and 2 mm for the lower optical system.
PMT detection
A photomultiplier is used for detection as the light intensities of the fluorescence can be very low.