Eppendorf AF2200 Plate Reader - Manual (Page 56)

Maintenance
Eppendorf
® PlateReader AF2200
English (EN)
56
Pipette 200 μL of 25 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells
according to the plate layout below.
Material/reagents
• 25 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)
• 0.01 M NaOH (=blank solution) (NaOH pellets)
• 96-well plate
• 200 μL pipette with tips
Calculating the detection limit (sensitivity)
The result of the detection limit formula specifies the sensitivity in pM units.
X 25 nM fluorescein
blank 0.01 M NaOH
Concentration
F
Concentration of fluorophore in pM units
Mean value
F
Mean RFU value of the wells filled with fluorophore
Mean value
B
Mean RFU value of the wells filled with the blank
solution
Std. deviation
B
Standard deviation of the RFU values of the wells
filled with the blank solution
( )
B
B F
F Stdev * 3 *
mean mean
ion Concentrat
imit DetectionL
−
=