Eppendorf AF2200 Plate Reader - Manual (Page 43)

Operation
Eppendorf ® PlateReader AF2200
English (EN)
43
6.4.5 MRW size
The MRW size determines the number of points to be measured in a well. Depending on the device and the
microplate type, a 1 x 1 area with a maximum of 15 x 15 points can be selected for the size. The diameter of
the individual measuring points is the same as the theoretically calculated diameter of the light beam in the
focus (see table).
Therefore, the MRW type shown in the software is only a schematic overview of the measuring pattern. The
pattern may vary when measuring real samples and the overlapping of the individual measuring points may
deviate slightly from the displayed pattern. Therefore, we recommend optimizing the parameters for
several measurements per well for each new application.
6.4.6 MRW limit
In addition to the size and type, a limit function allows the user to select a specific distance between the
light beam and the wall of the well (distance in μm). As previously mentioned, the software gives only a
schematic overview of the measuring pattern. The limit is calculated from the theoretical beam diameter of
the device. However, when measuring liquid samples, the diameter of the light beam is affected by the type
and volume of the liquid in the well.
The plate type (e.g., material of the microplate base) also affects the properties of the light beam. As a
result, the theoretical limit displayed in the software may not be identical to the actual limit when
measuring a real sample. Therefore, we highly recommend optimizing the parameters for several
measurements per well for each new application. Make sure that the selected limit guarantees an adequate
distance between the light beam and the wall of the microplate.
Measuring mode
Upper fluorescence intensity 2 mm
Lower fluorescence intensity 2 mm
Absorbance (microplate optics) 0.5 mm
Only a schematic overview of the measuring pattern is shown in the software. Therefore, the
parameters for several measurements per well must be optimized for every new application.
Make sure that the selected limit is sufficient to prevent an overlap between the light beam
and the wall of the microplate.
A value that is too low for the limit can lead to incorrect measuring results due to overlaps
between the light beam and the well wall.