Eppendorf AF2200 Plate Reader - Manual (Page 45)

Troubleshooting
Eppendorf ® PlateReader AF2200
English (EN)
45
7 Troubleshooting
7.1 General errors
Error Possible cause Remedy
Measuring results are
imprecise. • Reagent has exceeded its
shelf life.
 Ensure that the reagent is still within its
shelf life and properly prepared.
• Reagent not prepared
correctly.
 Use clean demineralized water of adequate
quality for preparation, if required.
• Pipetting incorrect.  Ensure that the pipette is calibrated and
that pipetting is being performed correctly.
• Incubation procedure
before measurement is
incorrect.
 If the method procedure requires
incubation before the measurement,
ensure that the temperature and time for
incubation are correctly observed.
• The microplate is not
completely filled with
measuring solution, and
contains bubbles.
 Ensure that the minimum microplate
volume required for a measurement is
reached and that there are no bubbles are
in the measuring solution.
• Turbidity of the
measuring solution.
 Centrifuge turbid measuring solutions
which contain particles and use the clear
supernatant.
• PlateReader is drifting.  Observe the ambient conditions.
 Prevent temperature changes.
 Contact Eppendorf Service.
• Plate compartment is
contaminated.
 Clean the plate compartment.
• Interfering substances
reinforce or weaken the
fluorescence signal.
 Remove the interfering substances.
• The plate compartment
flap is not closed.
 If the plate compartment flap is defective,
please contact Eppendorf Service.
Measuring results are
incorrect. • Method incorrectly
programmed.
 Ensure that the method parameters are
entered correctly.
• Standard solution not
prepared correctly.
 Ensure that the correct standard is used
and that the measuring solution for the
standard is prepared correctly.
Positioning cannot be
completed correctly. • The plate compartment is
heavily contaminated and
the spring mechanism is
not functioning correctly.
 Clean the plate compartment.
 Contact Eppendorf Service.