Eppendorf AF2200 Plate Reader - Manual (Page 55)

Maintenance
Eppendorf ® PlateReader AF2200
English (EN)
55
Calculating the precision from one measurement to the next
The result of the formula specifies the precision from one measurement to the next in % CV (coefficient of
variation).
8.6.3 Test instructions, lower fluorescence
The following tests can be conducted on the device to check the device specifications for this measuring
procedure:
• Sensitivity
• Precision within a plate
• Precision from one measurement to the next
Sensitivity
Conduct the measurement with the following settings to determine the detection limit for fluorescein.
Mean value
Well A1
Mean RFU value of well A1 over 20 kinetic cycles
Std. deviation
Well A1
Standard deviation of RFU values from well A1 over
the 20 cycles
Parameter Setting
Measuring mode Lower fluorescence
Wavelength, excitation 485 (20 nm)
Wavelength, emission 535 (25 nm)
Number of flashes 25
Integration time 40 (μs)
Setting time 0 (μs)
Signal gain Optimal
Plate type GRE96fb
wellA1
wellA1
mean
100 * stdev
(CV%) Precision =