Waters IC-Pak C Method Development Column - Manual (Page 6)

[ Care and Use ManUal ]
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
Waters IC-Pak C M/D Column 6
© 2007 Waters Corporation. Waters, The Science of What’s Pos-
sible, IC-Pak, Sep-Pak, and Guard-Pak are trademarks of Waters
Corporation. Millex, Milli-Q, and Durapore are trademarks of
Millipore Corporation.
November 2007 WAT036549 Rev 4 VW-PDF
Sales Offices:
Austria and European Export
(Central South Eastern Europe,
CIS and Middle East) 431 877 18 07
Australia 2 9933 1777
Belgium 32 2 726 1000
Brazil 55 11 5094 3788
Canada 800 252 4752
China 8621 6495 6999
CIS/Russia +7 495 3367000
Czech Republic 42 02 617 11384
Denmark 45 46 59 8080
Finland +358 9 5659 6288
France (33) 1 30 48 72 00
Germany 49 6196 400600
Hong Kong 852 29 64 1800
Hungary 36 1 350 5086
India and India Subcontinent
91 80 2 837 1900
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Norway 47 63 84 60 50
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b. Troubleshooting
Table 3: Column Problems and Solutions
VI. orderInG InForMAtIon
To order these parts, contact the nearest subsidiary (see back cover).
Table 4: Spare Parts and Accessories
Symptom Conditions Corrective Action
Excess pressure
buildup Filters plugged with
particulates. Check for
injector and pump seal
shedding. Replace filter.
Clogged tubing Unclog or replace tubing.
Failing injector Repair the injector.
Sample precipitates
on column Sample not soluble in
eluent Backflush column with
mobile phase for 30 min-
utes at 0.1 mL/min.
Use a compatible mobile
phase.
Loss of resolution,
broad peaks, low
plate counts Insufficient equilibration Continue equilibration
Incorrect diameter stain-
less steel tubing
Install 0.0009-inch stain-
less steel tubing.
Contaminated column Flush column with 100%
acetonitrile at 0.5 mL/min
for 30 minutes.
Part Number
IC-Pak C M/D Column, 3.9 x 150 mm WAT036570
Replacement frit for 3.9 mm column WAT015931
IC-Pak C M/D Guard-Pak™ Inserts, 10/pkg WAT098801