Waters IC-Pak C Method Development Column - Manual (Page 3)

[ Care and Use ManUal ]
Waters IC-Pak C M/D Column 3
Filtration
Before filtering the mobile phase, flush the filters with 100 mL of mobile
phase. Discard the filtered mobile phase. Filter and degas mobile phases
with a compatible Millex
® 0.45 µ filter for optimum long-term performance
of the column.
Use an in-line precolumn filter to remove particulates in the mobile phase
and contaminants which the 0.45 µm filter does not remove.
Glass Containers
Sodium leaching from glass viols causes artifacts when analyzing cations.
Use plastic containers for all solutions.
Organic Mobile Phases
Samples containing organic amines may exhibit hydrophobic interaction
between the mobile phase and packing. You may use a water-miscible
organic mobile phase, such as acetonitrile, as a modifier to reduce this.
Do not use methanol; over time it causes a significant decrease in cation
retention time. Pretreat the sample with a C1
8 Sep-Pak ® cartridge to
remove hydrophobic compounds.
Changeover to Mobile Phases Containing Salts
Perform changeover between on organic solvent and water containing
salts gradually to avoid salt precipitation. Use 18 megohm water as the
intermediate solvent. Do not exceed salt concentrations of 0.1 M.
Preparing the Mobile Phase
1. Put 800 mL of Milli-Q™ water into a one-liter volumetric flask.
2. Add 0.0292 g EDTA, free acid (reagent grade). Stir or place in ultra-
sonic bath for 15 minutes.
3. Add 189 µL Nitric Acid (Ultrex).
4. Dilute the solution to one liter with Milli-Q water and mix thoroughly.
5. Filter any undissolved EDTA from the solution and degas with one
of the following Millipore membranes (refer to Section ??? for part
numbers):
Aqueous Replacement Filters (0.45 µm, 47 mm)
Durapore
® Filters (0.22 µm, 47 mm)
b. Preparing the Sample Preparing
Sample cleanup with Sep-Pak cartridges prevents alteration of the column
chemistry by strongly adsorbing or precipitating sample components. Pass
the first few drops of sample to waste.
Filter prepared samples with a microporous filter to prevent excessive
pressure buildup due to particulate matter. Rinse the filter with 10 mL of
18 megohm water. Pass the first few drops of Sample through the filter
to waste.
IV. usInG tHe CoLuMn
a. Chromatography Guidelines
The life of a column is directly related to its care and use. Contamination
from samples and mobile phases and improper handling and storage
reduce column life.
Note: Before running the first analysis on the new column, perform the test
sample separation given in Section IV. b, Efficiency Testing.
Physical Limits
Maximum Pressure 27 MPa (27 atm or 4000 psi)
Flow Rote 1 m min
pH Range 2 to 7
Temperature:
Normal operation
Limits
25 °C
≤ 50 °C (do not freeze column)