Waters PAH Columns - Manual

[ Care and Use ManUal ]
Name of Document 1
Contents
I. IntroduCtIon
II. ConneCt Ing the Column to the hP lC Inst rument
a. Column Connectors and System Tubing Considerations
b. SLIPFREE Connectors
c. Minimization of Band Spreading
d. Measuring System Bandspread Volume
e. Measuring Gradient Delay Volume
f. Use of Narrow-Bore Columns (3.0 mm i.d.)
g. Impact of bandspreading on Column performance
(2.1 mm i.d. column)
h. System Modification Guidelines
III. Column equIlIbratIon
IV. Column usage
V. Column CleanIng, regeneratIng and storage
a. Cleaning and Regeneration
b. Storage
VI. t roubleshootIng
Waters Pah Column
I. IntroduCtIon
We are sure you will find that Waters built-in quality helps solve many of
your challenging separation problems. We strive to provide products with the
highest degree of lot-to-lot and column-to-column reproducibility to mini-
mize variations in your chromatographic results. Waters PAH columns are
manufactured and packed under highly controlled conditions. Each must pass
a series of stringent tests before being accepted for shipment. Included with
each column is the final Certificate of Analysis.
Polynuclear Aromatic Hydrocarbons (PAHs) are among the most frequently
monitored environmental contaminants. Standard and official methods for the
analysis of PAHs are found in compendia for air, drinking water, wastewater,
solid waste, and food analysis1.
Many of these methods specify HPLC, usually with UV and fluorescence detec-
tion, as the recommended analytical procedure.
Waters PAH columns are optimized for the HPLC analysis of PAHs. Figure 1
shows a chromatogram of 16 PAH compounds, listed as target pollutants by
the U.S. EPA. The Waters PAH columns achieve baseline resolution and excel-
lent peak symmetry for all 16 target analytes in less than 25 minutes, while
employing a simple water; acetonitrile binary gradient. The resolving power
of the PAH Columns provides superior peak identification and quantitation
for PAHs.
Florida Administrative Code 17.700 includes 2 additional compounds
(1-methyl naphthalene and 2-methyl naphthalene ) in addition to the 16 com-
pound EPA 610 mix that we currently use to show the proficiency of Waters
instrumentation to analyze PAH compounds. The new Waters PAH columns
resolve these two compounds along with the other 16, (see Figure 2).