Waters XBridge Peptide BEH C18 130A and 300A Columns - Manual (Page 7)

Figure 3. Parker Ferrule in a Waters Style Endfitting.
There is only one way to fix this problem: Cut the end of the tubing
with the ferrule, place a new ferrule on the tubing and make a new
connection. Before tightening the screw, make sure that the tubing
bottoms out in the endfitting of the column.
Conversely, if tubing with a Waters ferrule is connected to a
column with Parker style endfitting, the end of the tubing will
bottom out before the ferrule reaches its proper sealing position.
This will leave a gap and create a leak (Figure 4).
Note: The connection leaks if a Waters ferrule is connected to a
column with a Parker style endfitting.
Figure 4. Waters Ferrule in a Parker Style Endfitting.
There are two ways to fix the problem:
1. Tighten the screw a bit more. The ferrule moves forward, and
reaches the sealing surface. Do not overtighten since this may
end in breaking the screw.
2. Cut the tubing, replace the ferrule and make a new connection.
Alternatively, replace the conventional compression screw fitting
with an all-in-one PEEK™ fitting (Waters Part Number PSL613315)
that allows resetting of the ferrule depth. Another approach is to
use a SLIPFREE
® connector to always ensure the correct fit. The
fingertight SLIPFREE connectors automatically adjust to fit all
compression screw type fittings without the use of tools (Figure 5).
Figure 5. Single and Double SLIPFREE Connectors.
SLIPFREE connector features:
■ ■
Tubing pushed into endfitting, thereby guaranteeing a
void-free connection
■ ■
Connector(s) come(s) installed on tubing
■ ■
Various tubing i.d’s and lengths available
■ ■
Fingertight to 10,000 psi – Never needs wrenches
■ ■
Readjusts to all column endfittings
■ ■
Compatible with all commercially available endfittings
■ ■
Unique design separates tube-holding function from
sealing function
Table 5: Waters Part Numbers for SLIPFREE Connectors
Band Spreading Minimization
Figure 6 shows the influence of tubing internal diameter on
system band spreading and peak shape. As can be seen, the
larger tubing diameter causes excessive peak broadening and
lower sensitivity.
Figure 6. Effect of Connecting Tubing on System.
Gap
Diluted/Distorted Sample Band
0.005 inches
0.020 inches
0.040 inches
SLIPFREE Type Tubing Internal Diameter
Tubing Length 0.005” 0.010” 0.020”
Single 6 cm PSL 618000 PSL 618006 PSL 618012
Single 10 cm PSL 618002 PSL 618008 PSL 618014
Single 20 cm PSL 618004 PSL 618010 PSL 618016
Double 6 cm PSL 618001 PSL 618007 PSL 618013
Double 10 cm PSL 618003 PSL 618009 PSL 618015
Double 20 cm PSL 618005 PSL 618001 PSL 618017
Void
7
[ CARE AND USE MANUAL ]
XBridge Peptide BEH C
18
, 130Å and 300Å Columns