Waters XBridge Peptide BEH C18 130A and 300A Columns - Manual

CONT ENTS
I. GET TING START ED
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
II. COLUMN USE
a. Sample Preparation
b. Operating pH Limits
c. Solvents
d. Pressure
e. Temperature
III. SCALING UP/DOWN ISOC RATIC MET HODS
IV. T ROUBLESHOOTING
V. COLUMN CLEANING, REGENERATING
AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI. CONNECTING T HE COLUMN TO T HE HPLC
a. Column Connectors and System Tubing Considerations
b. Measuring System Bandspreading Volume
c. Measuring Gradient Delay Volume (or Dwell Volume)
VII. ADDITIONAL INFORMATION
a. Use of Narrow-Bore (3.0 mm i.d.)
b. Impact of Bandspreading Volume on 2.1 mm i.d.
Column Performance
c. Non-Optimized vs. Optimized LC-MS/MS System:
System Modification Recommendations
Thank you for choosing a Waters XBridge ® Peptide BEH C
18
, 130Å
or 300Å Column. The XBridge Peptide BEH C
18
, 130Å and 300Å
packing materials were designed to provide excellent peak shape,
high efficiency and excellent stability. The XBridge Peptide BEH C
18
,
130Å and 300Å packing materials are manufactured in a cGMP,
ISO 9002 certified planted using ultra pure reagent. Each batch
of XBridge Peptide BEH C
18
Column material has been qualified
with a peptide separation and the results are held to narrow
specification ranges to assure excellent, reproducible performance
for peptide separations. Every column is tested and a Performance
Test Chromatogram along with a Certification of Acceptance are
provided with each column.
XBridge Peptide BEH C
18
, 130Å and 300Å Columns
[ CARE AND USE MANUAL ]