Waters XBridge Peptide BEH C18 130A and 300A Columns - Manual (Page 3)

a. Sample Preparation
1. Sample impurities often contribute to column contamination.
One option to avoid this is to use Waters Oasis ® solid-phase
extraction cartridges/columns or Sep-Pak ® cartridges of the
appropriate chemistry to clean up the sample before analysis.
2. It is preferable to prepare the sample in the operating mobile
phase or a mobile phase that is weaker (less organic modifier)
than the mobile phase for the best peak shape and sensitivity.
3. If the sample is not dissolved in the mobile phase, ensure that
the sample, solvent and mobile phases are miscible in order to
avoid sample and/or buffer precipitation.
4. Filter sample with 0.2 µm filters to remove particulates. If
the sample is dissolved in a solvent that contains an organic
modifier (e.g., acetonitrile, methanol, etc.) ensure that the
filter material does not dissolve in the solvent. Contact the
filter manufacturer with solvent compatibility questions.
Alternatively, centrifugation for 20 minutes at 8,000 rpm,
followed by the transfer of the supernatant liquid to an
appropriate vial, could be considered.
b. Operating pH Limits
The recommended operating pH range for XBridge Peptide BEH
C
18
, 130Å and 300Å Columns is 1 to 12. A listing of commonly
used buffers and additives is given in Table 2. Additionally,
the column lifetime will vary depending upon the operating
temperature, the type and concentration of buffer used.
Column internal diameter (mm)
Column Length (mm) 1.0 2.1 4.6 10 19 30
50 0.04 0.17 0.83 3.9 14 35
100 0.08 0.35 1.7 7.8 28 70
150 0.12 0.52 2.5 12 42 106
250 - 0.87 4.2 20 70 176
Table 1: Empty Column Volumes in mL (multiply by 10 for flush solvent volumes)
II. COLUMN USE
To ensure the continued high performance of XBridge Peptide BEH C
18
, 130Å and 300Å Columns follow these guidelines:
3
[ CARE AND USE MANUAL ]
XBridge Peptide BEH C
18
, 130Å and 300Å Columns