Waters Peptide Separation Technology ACQUITY UPLC BEH130 and BEH300 - Manual (Page 6)

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February 2014 715001465 Rev C VW-PDF
T1
T8
T15
T5
T19
T12-T13
T12
T4
T9-T10
T10
T14
T13-T14
T19C
Minutes
6.00 9.00 12.00 15.00 18.00 21.00 24.00
*Chromatographic Conditions:
Column:
2.1 mm x 100 mm,
Sample: Tryptic digest of cytochrome c;
Flow rate: 0.20 mL/min
Mobile phase A: 0.045% TFA in water;
Mobile phase B: 0.045% TFA in acetonitrile;
Gradient:
from 0 to 15% B in 6 min, from 15 to
36% B in 20 min;
Temperature: 35 ˚C; Detection: UV 214 nm
VI. REP RESENTATIV E T EST CHROMATOGRAPH AND CONDITIONS FOR SEPARATION/
OF P ROT EIN DIGEST
Peak Identification
T1 N-AcGDVEK T8 TGPNLHGLFGR
T13-T14 KYIPGTK T15 MIFAGIK
T14 YIPGTK T5 CAQCHTVEK (heme attached)
T4 IFVQK T19 EDLIAYLK
T9-T10 KTGQAPGFSYTDANK T12-T13 GITWGEETLMEYLENPKK
T10 TGQAPGFSYTDANK T12 GITWGEETLMEYLENPK
T19C EDLIAY