Waters UPLC Intact Mass Analysis Application Kit - Manual (Page 5)

2. Plug the terminal block into terminal block I/O (terminal block
I/O is located on the back panel of the external divert valve).
3. Connect the other side of the red wire to pin #6 and black to the
pin #7 of a different terminal block as shown in table below.
4. Plug the terminal block into Switch II (Switch II is located on the
back panel of the ACQUITY UPLC Binary Solvent Manager [BSM]).
The divert valve can be programmed from the ACQUITY UPLC
inlet Method Editor in MassLynx. To set a salt divert time, open
inlet Method Editor. Then select Inlet> Events on the ACQUITY
UPLC BSM Instrument Method to open the Events dialog box. To
enable the external divert valve to be used as a divert valve, select
Run Events and enter the events in the events table by clicking
line 1 and 2. Table 2 depicts a salt divert event. When Switch 2 is
in ON position at 0.01 min, the solvent flow is diverted to waste
until 0.51 min, and then the valve is switched to allow the solvent
flow to enter the MS.
Table 2. ACQUITY UPLC BSM console of salt divert timetable/events.
1 (+)
2 (-)
Gradient Start In
3 (+)
4 (-)
Stop Flow In
5 Ground
6 (Red)
7 (Black)
Switch 2 Out
8
9
Switch 3 Out
10 Ground Out
11 (+)
12 (-)
0-2 V Analog 2 Out
V. CONNECTING T HE COLUMN TO WAT ERS SQ
DET ECTOR (SQD)
Follow steps 1-5 in Section II to attach the column to the pre
column tubing. Then follow Steps 18-23 below to connect the
column to the SQD.
1. Connect one end of the 22.20-inch long PEEK tube
(P/N 430001993) to the outlet of the column using a fitting
(P/N 410002276).
2. Connect the other end to the divert valve (Port 1) using another
fitting, as shown in the Figure 7. The divert valve is located at
the top-right corner of the SQD front panel.
3. Place the column in the column heater as shown in the Figure 6.
4. Connect one end of the 8.5-inch long PEEK tube (P/N
430001991) to Port 2 of the divert valve.
5. Connect the other end to the inlet of SQD sample probe (Figure 7).
6. Use a larger diameter PEEK tube (0.062 o.d. x 0.005 or
0.01-inch i.d.) for the waste line (not included in the kit).
Table 3. Salt divert timetable for SQD.
The divert valve can be programmed from the MS Method editor in
MassLynx. To set a salt divert time, open MS Method Editor . Then
select Option> Method events on the MS Method Editor to open the
Method events dialog box. To enable the valve to be used as divert
valve, select
Enable . This diverts the flow of solvent to waste for the
time period shown in the Method events timetable (0.5 min or user
defined value) then the valve is switched allowing the flow to go to
the MS (Table 3).
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UPLC Intact MASS Analysis Application Kit