Waters UPLC Intact Mass Analysis Application Kit - Manual (Page 2)

II. INSTALLING T HE P RE-COLUMN TUBING INTO
MASSP REP MIC RO DESALTING COLUMN
Required materials:
■ ■
1/4-inch open-end wrench
■ ■
5/16-inch open-end wrench
Figure 2. Installation of the pre-column tubing into the MassPREP Micro
Desalting Column.
Installation Procedure
1. Place the column on a flat surface (O-ring end facing up).
2. Remove the O-ring from the column.
3. Secure the column (P/N 186004032) with a 5/16-inch wrench
(bottom) as shown in Figure 2.
4. Tighten one end of the pre-column tubing (P/N 430001988)
into the column with a 1/4-inch wrench (a full turn beyond
hand-tight).
5. Connect the marked end (marked as solvent inlet) of the
column tubing assembly to the solvent outlet of the ACQUITY
UPLC
® Column Heater (Figure 3).
Note: Proceed from this point to either section III, IV, or V
depending upon the type of mass spectrometer you have.
Figure 3. Column assembly after attaching the column to the pre-column tubing.
III. CONNECTING T HE COLUMN TO LCT
P REMIER MS
Installation Procedure
1. Connect one end of the 15.04-inch long PEEK tube (P/N
430001992) to the outlet of the column using a fitting (P/N
410002276).
2. Connect the other end of the tubing to the salt divert valve
(Port 2) using another fitting, as shown in the Figure 4. The
salt divert valve is located at the top-left corner of the LCT
Premier™ front panel.
3. Place the column in the column heater as shown in the Figure 6.
4. Connect one end of the 8.5-inch long PEEK tube (P/N
430001991) to Port 1 of the divert valve using a fitting
5. Connect the other end to the inlet of LCT Premier sample probe
using another fitting (Figure 4).
6. Use a larger diameter PEEK tube (0.062 o.d. x 0.005 or
0.01-inch i.d.) for the waste line (not included in the kit).
Solvent Inlet Solvent Outlet
Flow
2
[ CARE AND USE MANUAL ]
UPLC Intact MASS Analysis Application Kit