Waters UPLC Intact Mass Analysis Application Kit - Manual (Page 3)

Figure 4. Fluidic configuration for LC-MS (UPLC-LCT Premier) analysis. A post-
column salt diversion valve (top-left corner of the LCT Premier) can be utilized
to divert buffers and nonvolatile salts to waste in the beginning of the LC/MS
analysis.
The divert valve can be programmed from the MS Method Editor in
MassLynx
® . To set a salt divert time, open MS Method Editor. Then
select Option> Solvent Delay on the MS Method Editor to open
the Solvent Delay dialog box. To enable the divert/injection valve
to be used as divert valve, select Enable Divert Valve. This diverts
the flow of solvent during a solvent delay period (0.5 min or user
defined value) either to, or away from, the source for the time
period shown in the solvent delay timetable (Table 1).
Table 1. Salt divert timetable.
IV. CONNECTING T HE COLUMN TO Q-TOF Premier
MS, AND SYNAPT HDMS
Neither Q-Tof Premier™ MS nor SYNAPT ® HDMS ® has an integrated
divert valve. However, an optional external 2 position valve (P/N
417000118) can be used with Q-Tof Premier and Synapt HDMS
mass spectrometers to divert buffers and nonvolatile salts present
in the sample to waste at the beginning of the LC/MS analysis.
Follow Steps 1–5 in Section II to attach the column to the pre
column tubing. Then follow Steps 12-17 below to connect the
column to the mass spectrometer.
1. Connect one end of the 15.04-inch long PEEK tube
(P/N 430001992) to the outlet of the column using a fitting
(P/N 410002276).
2. Connect the other end of the tubing to the external salt divert
valve (Port 4) using another fitting, as shown in the Figure 5.
3. Connect one end of the 8.5-inch long PEEK tube (P/N
430001991) to the external divert valve (Port 5).
4. Connect the other end to the inlet of MS sample probe (Figure 5).
5. Place the column in the column heater as shown in the Figure 6.
6. Use a larger diameter PEEK tube (0.062 o.d. x 0.005 or
0.01-inch i.d.) for the waste line (not included in the kit).
UPLC
Gradient
Column Heater
15.04”
8.5”
MS
Waste
UPLC
Gradient 15.04”
8.5”
MS
Waste
Position 1: Sample loading and salt divert to waste
Position 2: Sample elution to MS
3
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UPLC Intact MASS Analysis Application Kit