Waters Oasis Prospekt-2/Symbiosis Cartridges - Manual (Page 3)

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[ Care and Use ManUal ]
Oasis Prospekt-2/Symbiosis SPE Cartridges
Protocol for Oasis MCX and WAX Cartridges
Solvation: 100% methanol or acetonitrile
Flow rate: 5 mL/min
Volume: 1,000 µL
Equilibrate: 5% acetonitrile in 0.2% formic acid
Flow rate: 5 mL/min
Volume: 1,000 µL
Load: 5% acetonitrile in 0.2% formic acid
Flow rate: 1.0 mL/min
Inject: 20–100 µL of sample
Volume: 500 µL
Wash 1: 5% acetonitrile in 0.2% formic acid
Flow rate: 2 mL/min
Volume: 1,000–2,000 µL
Wash 2: 90% acetonitrile in 0.2% formic acid
Flow rate: 5 mL/min
Volume: 1,000–2,000 µL
Wash 3: 5% acetonitrile in 1% NH
4
OH
Flow rate: 5 mL/min
Volume: 1,000–2,000 µL
Elute: HPLC mobile phase
pH: >9.5
Flow rate: 0.5 mL/min
Protocol for Oasis MAX and WCX Cartridges
Solvation: 100% methanol or acetonitrile
Flow rate: 5 mL/min
Volume: 1,000 µL
Equilibrate: 5% acetonitrile in 1% NH
4
OH
Flow rate: 5 mL/min
Volume: 1,000 µL
Load: 5% acetonitrile in 1% NH
4
OH
Flow rate: 1.0 mL/min
Inject: 20–100 µL of sample
Volume: 500 µL
Wash 1: 5% acetonitrile in 1% NH
4
OH
Flow rate: 2 mL/min
Volume: 1,000–2,000 µL
Wash 2: 50% acetonitrile in 1% NH
4
OH
Flow rate: 5 mL/min
Volume: 1,000–2,000 µL
Wash 3: 5% acetonitrile in 0.2% formic acid
Flow rate: 5 mL/min
Volume: 1,000–2,000 µL
Elute: HPLC mobile phase
pH: <5.0
Flow rate: 0.5 mL/min
IV. orderIng InformatIon *
Description Format Particle Size Qty. Part No.
Oasis HLB Prospekt-2/Symbiosis cartridge 1 x 10 mm 30 µm 96/box 186005781
Oasis MCX Prospekt-2/Symbiosis cartridge 1 x 10 mm 30 µm 96/box 186005782
Oasis MAX Prospekt-2/Symbiosis cartridge 1 x 10 mm 30 µm 96/box 186005783
Oasis WCX Prospekt-2/Symbiosis cartridge 1 x 10 mm 30 µm 96/box 186005784
Oasis WAX Prospekt-2/Symbiosis cartridge 1 x 10 mm 30 µm 96/box 186005785
Oasis HLB Prospekt-2/Symbiosis cartridge 2 x 10 mm 30 µm 96/box 186005786
Oasis MCX Prospekt-2/Symbiosis cartridge 2 x 10 mm 30 µm 96/box 186004653
Oasis MAX Prospekt-2/Symbiosis cartridge 2 x 10 mm 30 µm 96/box 186004654
Oasis WCX Prospekt-2/Symbiosis cartridge 2 x 10 mm 30 µm 96/box 186004655
Oasis WAX Prospekt-2/Symbiosis cartridge 2 x 10 mm 30 µm 96/box 186004656
* For use with Spark Holland Prospekt-2/Symbiosis systems