Waters Oasis HLB Direct Connect HP 2.1 x 30 mm Column for Automated Sample Preparation with Waters UPLC with Online SPE Technology - Manual (Page 3)

[ Care and Use ManUal ]
Oasis HLB Direct Connect HP 2.1 x 30 mm Column 3
Waters Corporation
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trademarks of Waters Corporation.
720003351EN Rev A IH-PDF Feb 2010
Once the wash is completed, the next phase is the elution of the
trapped analyte from the extraction column on to the analytical
column. A suitable mobile phase for the example of basic pharma-
ceuticals is a gradient elution using 20 mM ammonium formate in
water, pH 3.2 for solvent A and 50/50 methanol/acetone with
20 mM ammonium formate, pH 3.2 for solvent B.
The selection of the best mobile phase will depend on the mix of
analytes and may require some method development on both the
loading/washing steps as well as the elution phase. Reconditioning
of the extraction column is critical for minimising any possible car-
ryover. It may be found that a re-condition with 100% methanol
is sufficient for this purpose. Complex matrices may require more
stringent re-conditioning, such as a mix of methanol, acetone and
hexane. This re-conditioning step and re-equilibration prior to the
next sample loading are completed during the parallel elution
phase of the second extraction column. Solvents that be used for
re-conditioning are:
• Water
• Methanol
• Isopropanol
• Acetonitrile
• Acetone
• Tetrahydrofuran
• Hexane
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All other countries: Waters Corporation U.S.A. 1 508 478 2000/1 800 252 4752
Description Part Number
Oasis HLB 20 µm 2.1 x 30 mm
Direct Connect HP Column
186005231
OA UPLC Online SPE Core System 176000835
OA UPLC Online SPE Startup Accessories 176000836
Column compartment Contact Customer Service
2777 or 2777C Autosampler Contact Customer Service