Waters Oasis HLB Direct Connect HP 2.1 x 30 mm Column for Automated Sample Preparation with Waters UPLC with Online SPE Technology - Manual

[ Care and Use ManUal ]
Oasis HLB Direct Connect HP 2.1 x 30 mm Column 1
The Oasis ® HLB Direct Connect HP 2.1 x 30 mm column makes it
possible to analyze specific analytes in a sample matrix when com-
bined with Waters ACQUITY
® columns and online SPE technology.
CONTENTS
I. INTRODUCTION
II. SAMPLE PREPARATION
III. EQUIPMENT
IV. TYPICAL CHROMATOGRAPHIC CONDITIONS
V. ORDERING INFORMATION
1. INTRODUCTION
Oasis HLB is a Hydrophilic-Lipophilic-Balanced, water-wettable, reversed-
phase sorbent for all your SPE needs. It is made from a specific ratio
of two monomers, the hydrophilic N-vinylpyrrolidone and the lipophilic
divinylbenzene. It provides superior reversed-phase capacity with a
neutral polar ‘hook’ for enhanced retention of polar analytes.
Water-wettable Oasis sorbents exhibit excellent retention capacity for a
wider polarity spectrum of analytes, even if the sorbent bed runs dry dur-
ing conditioning or sample loading. This means that your SPE methods will
be more rugged and robust, obviating the need for repeat preparation.
OaSIS HLB DIRECT CONNECT HP 2.1 x 30 mm COLUmN fOR aUTOmaTED
SamPLE PREPaRaTION wITH waTERS wITH UPLC ONLINE SPE TECHNOLOgy
The advantage of having higher retention capacity [k] is that more ana-
lytes are retained with fewer breakthroughs, improving the recovery and
overall reproducibility of your SPE method. The direct connect column
can be screwed directly into the switching valve or connected to fittings
like a conventional HPLC column.
II. SamPLE PREPaRaTION
Aqueous Samples: Water, Beverages
Adjust pH to maximize analyte retention on the SPE sorbent. Buffer
salts and dispersive agents may be used to increase partitioning onto
the SPE sorbent. Pretreatment to remove suspended matter prior to
SPE treatment may include filtration or centrifugation.
Non-Aqueous Liquid
When appropriate the sample may be diluted with aqueous buffers
and organic co-solvents for reversed-phased SPE. If sufficient dilution
has occurred, the sample may be treated in a manner similar to an
aqueous sample.
III. EQUIPmENT
Shown in Figure 1 is a schematic of Waters UPLC ® system with online
SPE technology, which consists of a 2777 high-pressure sample
manager, an ACQUITY UPLC
® quaternary solvent manager (QSM), an
ACQUITY UPLC binary solvent manager (BSM), two extraction columns,
an analytical column, and detection system (mass spectrometry, PDA/
eλPDA or fluorescence). The extraction columns are connected to a
switching valve so they can be operated in parallel.
2777c
Detector
ACQUITY
QSM
ACQUITY
BSM
SPE
Injection
LC
Injection
Extraction
Column A
ACQUITY BEH Column
Extraction
Column B
Waste
2.0 mL/min
0.4 mL/min
Valve A: Injection valve for online SPE LC-MS/MS
Valve B: switching valve for extraction columns
Valve C: injection valve for stand-alone LC-MS/MS
A
B C
Figure 1: Schematic of Waters
UPLC System with Online SPE
Technology.