Waters XBridge XP 2.5 µm Columns - Manual (Page 5)

XBridge XP 2.5 µm Columns 5
[ CARE AND USE MANUAL ]
VanGuard Pre-Column Installation Instructions
1. Remove the VanGuard Pre-Column from its box and shipping tube and remove the plastic plug.
2. Orient the pre-column so that the male end is facing up and carefully remove the black O-ring that holds the collet and ferrule in place during shipment
(collet and ferrule are not permanently attached).
3. Orient the XP 2.5 µm Column perpendicular to the work surface so that the column inlet is on the bottom.
4. From below, insert the VanGuard Pre-Column into the column inlet; turn the assembled column and pre-column 180° so that the pre-column is now on top.
5. Tighten with two 5/16” wrenches placed onto the XP 2.5 µm Column flats and VanGuard Pre-Column hex nut (male end) as shown in Figure 4.
6. While keeping pressure on the VanGuard Pre-Column against the XP 2.5 µm Column, tighten turn to set the collet and ferrule.
7. Check that the ferrule is set by loosening the connection and inspecting the ferrule depth.
8. Reattach the pre-column to the XP 2.5 µm Column, apply flow and inspect for leaks.
III. COLUMN USE
To ensure the continued high performance of XBridge XP 2.5 µm Columns, follow these guidelines:
a. Sample Preparation
1. Sample impurities and/or particulates often contribute to column contamination. One option to avoid column contamination is to use Waters Oasis ® or
Sep-Pak Solid-Phase Extraction (SPE) devices. To select the appropriate sorbent for a specific sample type, visit www.waters.com/sampleprep
2. It is preferable to prepare the sample in the initial mobile-phase conditions or a weaker solvent for the best peak shape and sensitivity.
3. If the sample is not prepared in the mobile-phase, ensure that the sample, solvent and mobile-phases are miscible in order to avoid sample and/or buffer
precipitation.
4. Filter sample with a 0.2 µm membrane to remove particulates. If the sample is dissolved in a solvent that contains an organic modifier (e.g., acetonitrile,
methanol, etc.) ensure that the membrane/filter material is compatible with the solvents in use. Alternatively, centrifuge the sample for 20 minutes at
8000 rpm, followed by the transfer of the supernatant to an appropriate vial could be considered.
b. pH Range
Table 4: Recommended pH Range
Chemistry pH Range
XBridge BEH C
18
1 - 12
XBridge BEH C
8
1 - 12
XBridge BEH Phenyl 1 - 12
XBridge BEH Shield RP18 2 - 11
XBridge BEH HILIC 1 - 9
XBridge BEH Amide 2 - 11
Column lifetime will vary depending on the combination of temperature, mobile-phase pH and
type of buffer/additive used. Table 5 lists the recommended buffers and additives for XBridge
XP 2.5 µm Columns.
Note: Working in combinations of extreme pH, temperature and pressure may result in reduced
column lifetime.