Waters XBridge XP 2.5 µm Columns - Manual (Page 10)

XBridge XP 2.5 µm Columns 10
[ CARE AND USE MANUAL ]
b. Troubleshooting Questions
1. Are you using 100% aqueous mobile-phases?
2. What is the age of the mobile-phase?
3. Is the mobile-phase filtered through a 0.2 µm membrane?
4. Was the mobile-phase prepared fresh or topped off?
5. Is the water source of adequate quality?
6. When was the last time the water system was serviced or was the bottle of water unopened?
7. Is bacterial growth a possibility (pH 7 phosphate buffer is susceptible to bacterial growth within 24 hours)?
8. If a neat standard is prepared in the initial mobile-phase conditions and injected, are the problems still observed?
9. If the sample is filtered/purified (i.e., SPE, filtration...etc.) is the problem still observed?
10. Has the quality of the samples changed over time?
c. Recommended Flow Rates and Anticipated Backpressures for Reversed-Phase XBridge XP 2.5 µm Columns
XP 2.5 µm, 2.1 mm ID Columns (40 °C)
Linear Velocity 3 mm/sec 4 mm/sec 5 mm/sec 6 mm/sec
Column Dimension
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
2.1 x 30 mm 0.45 1760 0.6 2350 0.75 2940 0.9 3520
2.1 x 50 mm 0.45 2640 0.6 3520 0.75 4400 0.9 5280
2.1 x 75 mm 0.45 3740 0.6 4980 0.75 6230 0.9 7470
2.1 x 100 mm 0.45 4830 0.6 6440 0.75 8055 0.9 9670
XP 2.5 µm, 3.0 mm ID Columns (40 °C)
Linear Velocity 3 mm/sec 4 mm/sec 5 mm/sec 6 mm/sec
Column Dimension
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
3.0 x 30 mm 0.9 2180 1.17 2840 1.53 3710 1.8 4360
3.0 x 50 mm 0.9 3040 1.17 3950 1.53 5170 1.8 6080
3.0 x 75 mm 0.9 4120 1.17 5350 1.53 7000 1.8 8230
3.0 x 100 mm 0.9 5190 1.17 6750 1.53 8825 1.8 10380
XP 2.5 µm, 4.6 mm ID Columns (40 °C)
Linear Velocity 3 mm/sec 4 mm/sec 5 mm/sec 6 mm/sec
Column Dimension
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
Flow Rate
[mL/min]
Backpressure
[psi]
4.6 x 30 mm 2.1 3360 2.8 4480 3.5 5600 4.2 6720
4.6 x 50 mm 2.1 4210 2.8 5620 3.5 7020 4.2 8430
4.6 x 75 mm 2.1 5280 2.8 7040 3.5 8800 4.2 10560
4.6 x 100 mm 2.1 6350 2.8 8460 3.5 10580 4.2 12700