Care and use manual, Acquity csh with cytochrome c ms conditions, Colum: acquity csh130 c – Waters Cytochrome c Digestion Standard User Manual
Page 4: See above figure for conditions, Cytochrome c digestion standard
T1
T13
-T14
T14
T4
T9
-T10
T10
T8
T15
T19C
T19
T12
-T13
T12
T5+Heme
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
1.1
1.2
2
3
4
5
6
7
8
9
10
11
12 min
T1
T13
-T14
T14
T4
T9
-T10
T10
T8
T15
T19C
T19
T12
-T13
T12
T5+Heme
AU
Colum:
ACQUITY CSH130 C
18
, 1.7 µm, 2.1 x 50 mm
Part number:
186006936
Mobile phase A: 0.1% Formic Acid in 100% Water
Mobile phase B: 0.085% Formic Acid in 75% Acetonitrile
Temp.:
40 °C
Wavelength:
214 nm
Reconstitution: 200 µL of Mobile Phase A
Injection Vol:
5 µL
Run Time:
15 minutes
Gradient:
Time
Flow Rate %A %B Curve
0.00 0.200
99.0 1.0 *
5.00 0.200
79.0 21.0 6
7.50 0.200
68.6 31.4 6
11.00 0.200
5.0 95.0 6
12.50 0.200
5.0 95.0 1
15.00 0.200
99.0 1.0 1
Column: XSelect CSH C
18,
5 µm
Mixer: 50 µL
T1 T13
-T14 T14
T4
T9
-T10
T10
T8
T15
T19C
T19
T12
-T13
T12
T5+Heme
2
3
4
5
6
7
8
9
10
11
12 min
AU
0.0
0.5
1.0
1.5
Column: ACQUITY CSH C
18,
1.7 µm
Mixer: 50 µL
T1 T13
-T14
T14
T4
T9
-T10
T10
T8
T15
T19C
T19
T12
-T13
T12
T5+Heme
2
3
4
5
6
7
8
9
10
11
12 min
AU
0.0
0.5
1.0
1.5
T1
T13
-T14
T14
T4
T9
-T10
T10
T8
T15
T19C
T19
T12
-T13
T12
T5+Heme
Column: ACQUITY CSH C
18,
1.7 µm
Mixer: 380 µL
2
3
4
5
6
7
8
9
10
11
12 min
AU
0.0
0.5
1.0
1.5
Intensity
0
2 x 10
9
4 x 10
9
6 x 10
9
2
3
4
5
6
7
8
9
10
11
12 min
Column: XSelect CSH C
18,
5 µm
Mixer: 50 µL
T1 T13
-T14
T14
T4
T9
-T10
T10
T8
T15
T19C
T19
T12
-T13
T12
T5+Heme
Column: ACQUITY CSH C
18,
1.7 µm
Mixer: 50 µL
T1 T13
-T14 T14
T4
T9
-T10
T10
T8
T15
T19C
T19
T12
-T13
T12
T5+Heme
Intensity
0
2 x 10
9
4 x 10
9
6 x 10
9
2
3
4
5
6
7
8
9
10
11
12 min
T1
T13
-T14
T14
T4
T9
-T10
T10
T8 T15
T19C
T19
T12
-T13
T12
T5+Heme
T1
T13
-T14
T14
T4
T9
-T10
T10
T8 T15
T19C
T19
T12
-T13
T12
T5+Heme
Column: ACQUITY CSH C
18,
1.7 µm
Mixer: 380 µL
Intensity
0
2 x 10
9
4 x 10
9
6 x 10
9
2
3
4
5
6
7
8
9
10
11
12 min
acQuIty cSh with cytochrome c Various conditions
typical digest using acQuIty cSh130 c
18
acQuIty cSh with cytochrome c mS conditions.
See above figure for conditions.
4
[ Care and Use ManUal ]
Cytochrome c Digestion Standard