Waters Cytochrome c Digestion Standard User Manual

Cytochrome c digestion standard, Care and use manual

Cytochrome c Digestion Standard

Cont entS

IntroductIon

II.

IdentIfyIng Parts

III.

usIng the Waters
extractIon ManIfold

IV.

needle tIP InstallatIon Procedure

sPare Parts and accessorIes

I. IntroductIon

The Cytochrome c Digestion Standard was prepared by digesting
Bovine Heart Cytochrome c (Uniprot, P/N P62894) with sequencing
grade trypsin. Each batch of digestion standard is prepared under
strict quality control criteria confirmed on the included certificate
of analysis. Protein digestion standards may be used for a variety
of applications, including evaluation and benchmarking of HPLC
and UPLC

Columns or evaluation of mass spectrometry and

chromatography instrumentation performance.

column

XBridge™ BEH300 C

XBridge BEH130 C

ACQUITY UPLC CSH™ and XSelect™

ACQUITY UPLC

BEH300 C

ACQUITY UPLC BEH130 C

Atlantis

Symmetry300™ C

BioSuite™ PA-B

BioSuite PA-A

II. Storage and StabIlIty

The lyophilized powder is stable at room temperature until the expiration
date printed on the label. Once reconstituted in 0.055% trifluoroacetic
acid in water, the solution is stable for one week when stored at 8 °C.
Stability can be extended by freezing samples at -20 °C.

III. reconStItutIon of the cytochrome c
dIgeStIon Standard for hPlc/uPlc

The protein digestion standards were prepared by digesting 1 nmol of
protein. For HPLC column evaluation, the recommended sample dilution
is 200 µl of 0.055% trifluoroacetic acid in water.

Table 1. Compatible chemistries that can be used with this standard.

[ Care and Use ManUal ]