Care and use manual, Typical digest using xbridge beh 130 c, Column – Waters Cytochrome c Digestion Standard User Manual
Page 3: Typical digest using an acquity uplc beh130 c, Typical digest using xbridge beh 300 c, Column: acquity uplc beh130 c, Column: xbridge beh300 c, Column: xbridge beh130 c
typical digest using xbridge beh 130 c
18
column
typical digest using an acQuIty uPlc beh300 c
18
column
AU
4
8
12
16
20
24
min
0.00
0.10
0.20
0.30
0.40
0.50
T1
T13T14 T14
T4
T9T10
T10
T19C
T15
T8
T5
T19
T12T13
T12
typical digest using an acQuIty uPlc beh130 c
18
column
AU
4
8
12
16
20
24
min
0.00
0.08
0.16
0.24
T1
T13T14
T14
T4
T9T10
T10
T19C
T8
T15
T5
T19
T12T13
T12
Column:
ACQUITY UPLC BEH130 C
18
, 2.1 x 100 mm, 1.7 μm
Part number:
186003555
Mobile phase A: 0.045% TFA in water
Mobile phase B: 0.045% TFA in acetonitrile
Temp.:
35 °C
Wavelength:
214 nm
Injection vol.:
5 µL
Run time:
61 minutes
Gradient:
Time Flow %A1 %B1 Curve
0.2 100 0
6 0.2 85 15 6
26 0.2 64 36 6
40 0.2 40 60 11
50 0.2 100 0 11
70 0 100 0 11
typical digest using xbridge beh 300 c
18
column
6
10
14
18
22
26
30
34
38 min
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
AU
T1
T13-
T14 and T14
T4
T9
-T
10
T10
T19C
T15
T8
T5
T19
T12-
T13
T12
Column:
XBridge BEH300 C
18
, 4.6 x 150 mm, 3.5 μm
Part number:
186003613
Mobile phase A: 0.055% TFA in water
Mobile phase B: 0.055% TFA in acetonitrile
Temp.:
35 °C
Wavelength:
214 nm
Injection vol.:
36 µL
Run time:
65 minutes
Gradient:
Time Flow %A %B Curve
0.96 100 0
9.9 0.96 85 15 6
42.9 0.96 64 36 6
45.0 0.96 40 60 11
50.0 0.96 100 0 11
83.0 0
100 0
11
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36 min
0.00
0.10
0.20
0.30
0.40
0.50
AU
T1
T13-
T14
T14
T4
T9
-T
10
T10
T19C
T8
T15
T5
T19
T12-
T13
T12
Column:
XBridge BEH130 C
18
, 4.6 x 150 mm, 3.5 μm
Part number:
186003569
Mobile phase A: 0.055% TFA in water
Mobile phase B: 0.055% TFA in acetonitrile
Temp.:
35 °C
Wavelength:
214 nm
Injection vol.:
36 µL
Run time:
65 minutes
Gradient:
Time Flow %A %B Curve
0.96 100 0
9
0.96 85 15 6
39 0.96 64 36 6
43 0.96 40 60 11
58 0.96 100 0 11
67 0
100 0 11
Column
ACQUITY UPLC BEH300 C
18
, 2.1 x 100 mm, 1.7 μm
Part number:
186003686
Mobile phase A: 0.055% TFA in water
Mobile phase B: 0.055% TFA in acetonitrile
Temp.:
35 °C
Wavelength:
214 nm
Injection vol.:
5 µL
Run time:
61 minutes
Gradient:
Time Flow %A1 %B1 Curve
0.2 100 0
6.6 0.2 85 15 6
28.6 0.2 64 36
6
30.0 0.2 40 60
11
40.0 0.2 100 0
11
62.0 0
100 0
11
3
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Cytochrome c Digestion Standard