Waters XSelect CSH HPLC Columns - Manual (Page 7)

[ CARE AND USE MANUAL ]
7 XSelect CSH HPLC Columns
There are two ways to fix the problem:
1. Tighten the screw a bit more. The ferrule moves forward, and reaches
the sealing surface. Do not over tighten since this may end in breaking
the screw.
2. Cut the tubing, replace the ferrule and make a new connection.
Alternatively, replace the conventional compression screw fitting
with an all-in-one PEEK fitting (Waters part number PSL613315)
that allows resetting of the ferrule depth. Another approach is to
use a SLIPFREE
® connector to always ensure the correct fit. The
fingertight SLIPFREE ® connectors automatically adjust to fit all
compression screw type fittings without the use of tools (Figure 5).
Figure 5: Single and Double SLIPFREE ® Connectors
SLIPFREE ® Connector Features:
• Tubing pushed into endfitting, thereby guaranteeing a void-free
connection
• Connector(s) come(s) installed on tubing
• Various tubing IDs and lengths available
• Fingertight to 10,000 psi – never needs wrenches
• Readjusts to all column endfittings
• Compatible with all commercially available endfittings
• Unique design separates tube-holding function from sealing function
Table 5: Waters Part Numbers for SLIPFREE ® Connectors
Band Spreading Minimization
Figure 6 shows the influence of tubing internal diameter on system
band spreading and peak shape. As can be seen, the larger tubing
diameter causes excessive peak broadening and lower sensitivity.
Figure 6: Effect of Connecting Tubing on System
b. Measuring System Band-spreading Volume and
System Variance
This test should be performed on an HPLC system with a single
wavelength UV detector (not a Photodiode Array (PDA)).
1. Disconnect column from system and replace with a zero dead
volume union.
2. Set flow rate to 1 mL/min.
3. Dilute a test mix in mobile phase to give a detector sensitivity of
0.5 - 1.0 AUFS (system start up test mix can be used which contains
uracil, ethyl and propyl parabens; Waters part number WAT034544).
4. Inject 2 to 5 µL of this solution.
SLIPFREE ® Type Tubing Internal Diameter
Tubing Length 0.005” 0.010” 0.020”
Single 6 cm PSL 618000 PSL 618006 PSL 618012
Single 10 cm PSL 618002 PSL 618008 PSL 618014
Single 20 cm PSL 618004 PSL 618010 PSL 618016
Double 6 cm PSL 618001 PSL 618007 PSL 618013
Double 10 cm PSL 618003 PSL 618009 PSL 618015
Double 20 cm PSL 618005 PSL 618001 PSL 618017
Diluted/Distorted Sample Band
0.005 inches
0.020 inches
0.040 inches