Waters Extraction Manifold - Manual (Page 3)

3
[ Care and Use ManUal ]
Extraction Manifold
Oasis™ HLB
Cartridge
Needle Tip
(polypropylene)
Attention: Proper use of many SPE cartridges requires that the liquid level does not fall below the top frit. This is
especially true for Steps a and b listed above. Close each valve, as necessary, so as to prevent the cartridge from
drying out. Otherwise poor recovery and/or reproducibility may result. (See references on Waters website: www.
waters.com/oasis). This precaution is not necessary when using Waters Oasis HLB Extraction Cartridges.
Figure 2. Waters Sep-Pak ® Cartridges
and most SPE devices are firmly
seated into the Luer hubs atop valves.
(Sep-Pak Classic Cartridges require
male-male Luer adapters for this
purpose.) Valve (stopcock) handle is
rotated to an open or closed position
(as indicated) to control the flow
through an individual cartridge.
Figure 3. Waters Oasis HLB Extraction
Cartridges are fitted directly to the
needle tips. Note: No valves (stopcocks)
are needed.
8. To install or remove a rack of collection vessels, first close the exit valve (the pump can stay on;
alternatively, turn off the pump). Break the vacuum in the manifold changer completely by slightly
offsetting the white ball from its seal (see Figure 4). Then remove the cover. After a rack as been
installed or removed, replace the cover with the corresponding notches on the top plate of the rack. Then,
open the exit valve (and, if you had turned it off, turn on the vacuum pumps) to re-evacuate the chamber.
Vacuum may be temporarily increased by putting your finger over the bleed port hold at the end of the vacuum
control valve (see Figure 4). Once fluid flow begins, release your finger to restore preset vacuum level.
Sep-Pak ® Vac
Cartridge
Valve (stopcock)
OPEN CLOSED