Waters Extraction Manifold - Manual (Page 2)

2
[ Care and Use ManUal ]
Extraction Manifold
II. IdentIfyIng Parts
Before assembling the Waters Extraction Manifold, ensure that you
have these parts (refer to Figure 1 on previous page):
In Extraction Manifold Package:
■ ■
Glass chamber with exit valve installed
■ ■
White Delrin cover* with polyethylene gasket,
vacuum gauge, control and release valves installed
■ ■
Needle tips, long (polypropylene), 20/pk
■ ■
Luer plugs, male (polypropylene), 25/pk
■ ■
Needle tip ejector tool (not shown)
Packaged separately:
■ ■
Rack, 20-position, as specified
*The three posts on the underside of the cover have two purposes. First, they
serve as elevated feet so that the cover can be set down conveniently on the
bench top without damaging the needle tips that protrude through the top of the
cover. Second, they align only one way with the corresponding notches in the
top plate on a rack. This makes is easier to keep track of a particular sample/
cartridge and its corresponding collection vessel.
caution
■ ■
Glass vessels under vacuum have a danger of implosion if
struck or damaged.
■ ■
Inspect the glass chamber for cracks, scratches, or other
defects prior to each use.
■ ■
Do not use a chamber with visible defects.
■ ■
Do not exceed a vacuum level of 22” Hg (560 mm Hg).
III. usIng the Waters extractIon ManIfold
A typical solid-phase extraction (SPE) procedure consists of the
following steps:
a. Condition/equilibrate the cartridge bed.
b. Load the sample.
c. Wash off interferences.
d. Elute analyte(s) of interest.
The Waters Extraction Manifold permits these steps to be carried
out on up to twenty cartridges in parallel. Figure 1 shows the
manifold assembled (without cartridges or valves) with a rack of
tubes in place ready to collect the eluates in Step d. In Steps a–d,
a rack is not necessary as the eluates from these steps normally
are treated as waste. The waste is drawn down into a liquid trap
through the exit valve at the base of the glass chamber. However,
when optimizing or troubleshooting an SPE protocol, it may be
desirable to collect fractions in Steps b, c, and d; in this case, a
separate set of tubes will be used for each of these steps.
Procedure
1. Connect the vacuum source to the outlet of a liquid trap using
vacuum tubing. Connect the trap inlet to the exit valve at the
case of the glass chamber using 3/8” I.D. vacuum tubing.
Attention: Make certain that the liquid trap is compatible with, and
has sufficient capacity to collect, all the waste that will be generated
in your SPE procedure. If the trap capacity is too small, waste may
contaminate and/pr damage the vacuum pump.
2. Put the cover on the glass chamber.
3. Install a value (stopcock) in each of the 20 ports with the valve
handle pointing outward, perpendicular to the edge of the
chamber, so it is easily accessible for manipulation. Turn the
body of each valve slightly to seal its needle tip firmly in its
hold. The tips will extend into the mouths of the corresponding
collection vessels, once a rack is in place underneath the cover.
4. Put an extraction cartridge into each valve hub and seat it firmly.
5. Close all valves by turning handles until each indicator shows
that valve is in the closes position. See figure 2.
When using Waters Oasis ® HLB Cartridges, stopcocks are not needed.
Instead, install a needle tip with female Luer hub into each of the 20
ports. Make sure that the ridges on the outside of a needle tip (at the
top just under the hub) mate with the grooves inside the hold in the
cover. Seal all unused tips with make Luer plugs. Put an Oasis HLB
Cartridge into each unplugged needle tip hub. See Figure 3.
6. Turn on the vacuum pump. Adjust control valve to set the
desired vacuum level.
7. Follow SPE protocol recommended for the type of cartridge
used and the application being done. Add fluid into all
cartridge reservoirs for each step before opening the valves
to start or resume flow.