Waters Dye QCRM Kit - Manual (Page 6)

Dye Quality Control Reference Material Kit 6
[ CARE AND USE MANUAL ]
1. From the shortcut bar, click FractionLynx > Collection
Control, and verify that your system is activated.
2. From the MassLynx toolbar, click Start.
UV Chromatography Dye Test Results
These chromatograms represent typical results of the dye test. The
chromatograms show three separate color samples of blue, yellow,
and violet collected into separate vials labeled 1:16, 1:17, and 1:18
to 1:19.
Requirements: Sample dye kit, which contains three compounds for
testing the ability of the AutoPurification System to perform high-flow-
rate targeting and fractionation of specified masses and wavelengths.
The compounds elute in this order:
• Thionin acetate loses the acetate salt (C
2
H
4
O
2
) to give this [M+H] ion:
Thionin ([M+H] = 228.1), lambda max. = 590 nm
• • Thioflavin T loses a Cl to give this [M+H] ion:
Thioflavin T ([M+H] = 283.1), lambda max. = 418 nm
• • Crystal violet loses HCl to give this [M+H] ion:
([M+H] = 372.2), lambda max. = 590 nm
Tip: Report masses to one decimal place.
This sample was collected using the Waters 2998 PDA Detector. The wavelength values
were put in the sample list, and the fraction triggers were set to Wavelength A and B.
• The extracted wavelength shows the correct picture of what
was actually collected.
• Click Display > Wavelength to input the wavelengths of
choice, and then click OK to get the extracted wavelengths.
• To extract the spectrum, double-click the peak in the Total
Absorbance Chromatogram (TAC).
Actual results can vary if different column sizes, flow rates, and
injection volumes are used.
MS Chromatography Dye Test Results
The chromatograms above represent typical results of the dye test. The three
components of the dye mix have been separated and collected into separate vials
labeled 1:16, 1:17, and 1:18 to 1:19.
The extracted ion shows the correct picture of what was actually
collected into each vial. Double-click the peak in the Total Ion
Chromatogram (TIC) to extract the spectrum, and double-click the
spectrum of the mass of interest to extract the mass chromatograms.
Always consider the extracted ion or wavelength when trouble-
shooting as it will give the correct representation of what was
actually collected.
IV. TROUBLESHOOTING
For specific troubleshooting of the system and settings refer to the
AutoPurification System Guide 71500135202ra.