Care and use manual – Waters Oasis Mixed-Mode Ion-Exchange Cartridges and 96-Well Plates User Manual
Page 5
VI. OASIS 2X4 MET HOD OPTIMIZED FOR
µELUTION PLAT E
The proven Oasis 2x4 Method elution solvent is optimized to
accommodate the elutropic requirement of the small elution
volume. Methanol is good as a generic elution solvent, but is
often not strong enough for 25 µL elution volumes. The elution
solvent recommended to be used with the μElution plate must
possess a high enough elutropic strength to fully elute analytes
in small volumes, and be appropriate for a diverse set
of analytes.
The recommended elution solvent for the Oasis 2x4 Method
optimized for the µElution plate format is 60% CH
3
CN:40%
CH
3
OH with a modifier. This was chosen as a starting point as
it meets all of the above criteria.
Note: In order to ensure optimal performance with plasma samples,
a small amount of the equilibrium solution should remain on the
top of the bed prior to loading.
For more information, download Oasis Sample Extraction Products
brochure (literature Code
n www.waters.com.
VII. OASIS PST µELUTION MET HOD OPTIMIZED
FOR T HERAPEUTIC PEPTIDES BIOANALYSIS
The bioanalysis of therapeutic peptides <5,000 Dalton currently
presents analytical challenges for the pharmaceutical industry.
Typically, regulatory compliant bioanalytical methods are
routinely required to achieve LLOQs in the low pg/mL range
in biological matrices, and must demonstrate linear response
over several orders of magnitude. Waters offers the new PST
Therapeutic Peptide Method Development Kit and protocol that
simplifies the process of sample preparation and LC method
development for the analysis of therapeutic peptides in plasma.
The kit contains an Oasis PST µElution Method Development Plate,
a PST reversed-phase column and a detailed screening protocol.
Kindly, refer to the detailed protocol in the PST Therapeutic
Peptide Method Development Kits for the necessary details
needed to screen for a bioanalytical method, that is capable of
achieving the required sensitive and accurate quantification of
therapeutic peptides <5,000 Dalton.
For more information, download Bioanalysis Method Development
Strategy for Therapeutic Peptides (Literature code
at
VIII. OASIS 96 W ELL PLAT ES
All Oasis 96-Well Plates, including µElution plate are designed to
work individually as well as on any automated robotics system.
The specific dimensions are:
96-Well Plate:
■
■
Length = 5.030 inches
■
■
Width = 3.365 inches
■
■
Height = 2.015 inches
µElution Plates:
■
■
Length = 5.030 inches
■
■
Width = 3.365 inches
■
■
Height = 1.501 inches
All Waters Oasis 96 Well Plates and µelution plates meet
the ANSI (American National Standards Institute) guidelines
for microplates.
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[ CARE AND USE MANUAL ]
Oasis Mixed-Mode Ion-Exchange Cartridges and 96-Well Plates