Waters Oasis Mixed-Mode Ion-Exchange Cartridges and 96-Well Plates - Manual (Page 4)

Table 1: Recommended Volume for Generic Methods (assuming 1:1 dilution)
Cartridges 96-well plate µElution Plate
Cartridges size/
Sorbent mass
1 cc 3 cc 6 cc 12 cc 20 cc 35 cc 5 mg 10 mg 30 mg 60 mg 2 mg
Condition/
Equilibration (ml)
1 ml 2 ml 3 ml 5 ml 10 ml 50 ml 0.2 ml 0.5 ml 0.5 -1.0 ml 0.5 -2.0 ml 0.2 ml
Maximum load of
matrix and dilution
1 ml 2 ml 5 ml 15 ml 30 ml 100 ml 0.5 ml 1 ml 1-2 ml 1-2 ml 0.025-0.75 ml
Wash (ml) 1 ml 2 ml 4 ml 5 ml 10 ml 40 ml 0.2 ml 0.5 ml 0.5 -1.0 ml 1 - 2 ml 0.2 ml
Elute (ml) 1 ml 2 ml 4 ml 5 ml 10 ml 60 ml 0.05-0.2 ml 0.15-0.3 ml 0.4-1.0 ml 0.8-2.0 ml 0.025-0.10 ml
Note: Above listed sample volumes are for biological samples; for certain types of samples (i.e. drinking water) up to 20 times of sample solution is possible.
Note: Solution should be made fresh daily.
Table 2: Load Volumes for Large Volume WATER Analysis
Cartridge size/Sorbent mass 1 cc 3 cc 6cc (200 mg, 30 µm) 6cc (200 mg, 60 µm) 12 cc 20 cc 35 cc
Load (ml water*) (total of
matrix and dilution)
50 ml 200 ml 500 ml 1000 ml 1000 ml 2000 ml 5000 ml
V. OASIS SORBENT SELECTION AND P ROTOCOL CHART
The Oasis 2x4 Method is a simple, logical approach to the
selection of an SPE sorbent and protocol. Two protocols and
four sorbents provide the flexibility to extract acids, bases,
and neutrals with high SPE recoveries while removing matrix
components that may interfere with analysis.
Follow the simple steps outlined in this flow chart to achieve
high recoveries and the cleanest extracts:
■ ■
Characterize your analyte [Neutral, Acid or Base, pK
a
]
■ ■
Select one of the four Oasis Sorbents
■ ■
Apply the indicated Protocol [1 or 2]
■ ■
Determine SPE recoveries by LC analysis
*Recoveries for all acids, bases and neutral compounds
are greater than 85% with RSD’s less than 5%.
Strong
Acids
Weak
Acids
Weak
Bases
Strong
Bases
Weaker
Acids
Neutrals
Weaker
Bases
Oasis 2x4 Strategy
Only 2 protocols and 4 sorbents to
analyze all types of compounds:
acids, bases, and neutrals
For Weak Bases
pKa 2–10:
Use Oasis MCX
For Weak Acids
pKa 2–8:
Use Oasis MAX
For Strong Acids
pKa <1:
Use Oasis WAX
For Strong Bases
pKa >10:
Use Oasis WCX
Prepare Sample Prepare Sample
Condition, Equilibrate,
and Load Sample
Condition, Equilibrate,
and Load Sample
Wash:
2% HCOOH
Wash:
5% NH
4
OH
Elute 1:
100% CH
3
OH
Elute 1:
100% CH
3
OH
Elute 2: 5% NH
4
OH
in CH
3
OH
Elute 2: 2% HCOOH
in CH
3
OH
Apply Protocol 1 Apply Protocol 2
4
[ CARE AND USE MANUAL ]
Oasis Mixed-Mode Ion-Exchange Cartridges and 96-Well Plates