Waters Oasis Mixed-Mode Ion-Exchange Cartridges and 96-Well Plates - Manual (Page 3)

15. Switch off the vacuum pump or stop vacuum by closing the
valve. (before switching off the vacuum pump, please reduce
the vacuum to the lowest possible).
16. Release vacuum and discard waste fluids, insert collection
device, and replace the cover.
17. Apply 5% ammonium hydroxide in methanol as elution
solvent and let it flow through by gravity before switching on
the vacuum pump.
18. Switch on or open valve at lowest possible vacuum and
gradually increase as needed.
19. Pull vacuum for another 30 sec to a minute (to collect all
elution solvent).
20. Remove collection device.
21. Evaporate/Reconstitute or Dilute as needed.
22. Transfer to vial or plate for analysis.
23. If using plates, cover prior to analysis.
Note: For the load and elute steps, it is recommended that flow
rates should not exceed 1.0 ml/min. In all other steps, flow rates up
to 5 ml/min are acceptable.
Note: You may need to momentarily increase the vacuum to start
the flow of aqueous solutions.
IV. SOLID PHASE EX T RACTION FOR ACIDIC
COMPOUNDS USING OASIS MAX AND
ST RONGLY BASIC COMPOUNDS USING
OASIS WCX
1. Place Oasis MAX or Oasis WCX Cartridge or Plate on the
vacuum manifold and set the vacuum to 5” Hg.
2. Condition with Methanol.
3. Equilibrate with Water
a. In each case (conditioning and equilibration) add the
solvent before applying vacuum.
4. Switch off the vacuum pump or Stop vacuum by closing the
valve (before switching off the vacuum pump, please reduce
the vacuum to the lowest possible).
5. Load your diluted sample.
6. Switch on or open valve at lowest possible vacuum and
gradually increase as needed.
7. Switch off the vacuum pump or stop vacuum by closing the valve.
8. Apply 5% ammonium hydroxide in water as wash solvent.
9. Switch on vacuum to 5”Hg (adjust/increase as needed).
10. Pull vacuum for another 30 sec to a minute to eliminate
residual wash solvent.
11. Switch off the vacuum pump or stop vacuum by closing the
valve. (before switching off the vacuum pump, please reduce
the vacuum to the lowest possible).
12. Apply 100% organic elution solvent and let it flow through
by gravity before switching on the vacuum pump.
13. Switch on vacuum to 5”Hg (adjust/increase as needed).
14. Pull vacuum for another 30 sec to a minute to eliminate
residual wash solvent.
15. Switch off the vacuum pump or stop vacuum by closing the
valve. (before switching off the vacuum pump, please reduce
the vacuum to the lowest possible).
16. Release vacuum and discard waste fluids, insert collection
device and replace the cover.
17. Apply 2% formic acid in methanol as elution solvent
and let it flow through by gravity before switching on the
vacuum pump.
18. Switch on or open valve at lowest possible vacuum and
gradually increase as needed.
19. Pull vacuum for another 30 sec to a minute (to collect all
elution solvent).
20. Remove collection device.
21. Evaporate/Reconstitute or Dilute as needed.
22. Transfer to vial or plate for analysis.
23. If using plates, cover prior to analysis.
Note: For the load and elute steps, it is recommended that flow
rates should not exceed 1.0 ml/min. In all other steps, flow rates up
to 5 ml/min are acceptable.
Note: You may need to momentarily increase the vacuum to start
the flow of aqueous solutions.
3
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Oasis Mixed-Mode Ion-Exchange Cartridges and 96-Well Plates