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Care and use manual, What flow rate can i use with each type of emitter – Waters ACQUITY UPLC M-Class Columns User Manual

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[ CARE AND USE MANUAL ]

What flow rate can I use with each type of emitter?

The flow rate of an LC method should be determined by the

optimal flow rate for the particular column. The optimal flow rate
is directly related to the column I.D.

A PicoTip is ideally suited for nanospray applications while the

TaperTip is suited for microspray applications (>1 µL/min).

Waters currently does not recommend the use of a nanospray

source for the 300 µm column format.

1

Trap columns designed for 1D (low pH only) work. These 1D traps are reversible.

2

Trap columns designed for 2D (high pH/low pH) work. These 2D traps are not reversible.

VIII. LONG T ERM ACQUIT Y UPLC M-CLASS
COLUMN STORAGE

When not in use, it is recommended to maintain flow through

the ACQUITY UPLC M-Class Column using 100% eluent B to
maximize bed stability and minimize potential bed drying.

If prolonged column storage is required or if the column needs

to be removed from the system, it is recommended that the
ACQUITY UPLC M-Class Column be flushed with 100% organic
solvent containing no TFA or FA (e.g., simply store in
100% acetonitrile).

ACQUITY UPLC M-Class Column Care & Use

IX. ORDERING INFORMATION

ACQUITY UPLC M-Class Columns

Product Description

Particle Size

Dimensions

Part Number

ACQUITY UPLC M-Class BEH C

18

, 130Å

5 µm

300 µm x 50 mm

186007471

ACQUITY UPLC M-Class HSS T3

1.8 µm

300 µm x 150 mm

186007472

ACQUITY UPLC M-Class HSS T3

1.8 µm

75 µm x 150 mm

186007473

ACQUITY UPLC M-Class HSS T3

1.8 µm

75 µm x 250 mm

186007474

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

75 µm x 100 mm

186007475

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

75 µm x 150 mm

186007476

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

75 µm x 200 mm

186007477

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

75 µm x 250 mm

186007478

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

100 µm x 100 mm

186007479

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

150 µm x 100 mm

186007480

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

150 µm x 50 mm

186007513

ACQUITY UPLC M-Class CSH C

18

, 130Å

1.7 µm

150 µm x 150 mm

186007514

ACQUITY UPLC M-Class BEH C

18

, 130Å

1.7 µm

75 µm x 100 mm

186007481

ACQUITY UPLC M-Class BEH C

18

, 130Å

1.7 µm

75 µm x 150 mm

186007482

ACQUITY UPLC M-Class BEH C

18

, 130Å

1.7 µm

75 µm x 200 mm

186007483

ACQUITY UPLC M-Class BEH C

18

, 130Å

1.7 µm

75 µm x 250 mm

186007484

ACQUITY UPLC M-Class BEH C

18

, 130Å

1.7 µm

100 µm x 100 mm

186007485

ACQUITY UPLC M-Class BEH C

18

, 130Å

1.7 µm

150 µm x 100 mm

186007486

ACQUITY UPLC M-Class BEH C

18

, 300Å

1.7 µm

75 µm x 100 mm

186007487

ACQUITY UPLC M-Class BEH C

18

, 300Å

1.7 µm

100 µm x 100 mm

186007488

ACQUITY UPLC M-Class BEH C

18

, 300Å

1.7 µm

150 µm x 100 mm

186007489

ACQUITY UPLC M-Class BEH C

18

, 300Å

1.7 µm

75 µm x 150 mm

186007490

ACQUITY UPLC M-Class BEH C

18

, 300Å

1.7 µm

75 µm x 200 mm

186007491

ACQUITY UPLC M-Class BEH C

18

, 300Å

1.7 µm

75 µm x 250 mm

186007492

ACQUITY UPLC M-Class BEH C4, 300Å

1.7 µm

75 µm x 100 mm

186007493

ACQUITY UPLC M-Class BEH C4, 300Å

1.7 µm

100 µm x 100 mm

186007494

ACQUITY UPLC M-Class BEH C4, 300Å

1.7 µm

150 µm x 100 mm

186007495

ACQUITY UPLC M-Class Symmetry C

18

Trap, 100Å

5 µm

180 µm x 20 mm

186007496

1

ACQUITY UPLC M-Class Symmetry C

18

2D Trap, 100Å

5 µm

180 µm x 20 mm

186007497

2

ACQUITY UPLC M-Class Symmetry C

18

Trap, 100Å

5 µm

300 µm x 50 mm

186007498

1

ACQUITY UPLC M-Class Symmetry C

18

2D HCP Trap, 100Å

5 µm

300 µm x 25 mm

186007499

2

ACQUITY UPLC M-Class Symmetry C

18

V/V Trap, 100Å

5 µm

180 µm x 20 mm

186007500

1