Care and use manual, What flow rate can i use with each type of emitter – Waters ACQUITY UPLC M-Class Columns User Manual
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[ CARE AND USE MANUAL ]
What flow rate can I use with each type of emitter?
The flow rate of an LC method should be determined by the
optimal flow rate for the particular column. The optimal flow rate
is directly related to the column I.D.
A PicoTip is ideally suited for nanospray applications while the
TaperTip is suited for microspray applications (>1 µL/min).
Waters currently does not recommend the use of a nanospray
source for the 300 µm column format.
1
Trap columns designed for 1D (low pH only) work. These 1D traps are reversible.
2
Trap columns designed for 2D (high pH/low pH) work. These 2D traps are not reversible.
VIII. LONG T ERM ACQUIT Y UPLC M-CLASS
COLUMN STORAGE
When not in use, it is recommended to maintain flow through
the ACQUITY UPLC M-Class Column using 100% eluent B to
maximize bed stability and minimize potential bed drying.
If prolonged column storage is required or if the column needs
to be removed from the system, it is recommended that the
ACQUITY UPLC M-Class Column be flushed with 100% organic
solvent containing no TFA or FA (e.g., simply store in
100% acetonitrile).
ACQUITY UPLC M-Class Column Care & Use
IX. ORDERING INFORMATION
ACQUITY UPLC M-Class Columns
Product Description
Particle Size
Dimensions
Part Number
ACQUITY UPLC M-Class BEH C
18
, 130Å
5 µm
300 µm x 50 mm
186007471
ACQUITY UPLC M-Class HSS T3
1.8 µm
300 µm x 150 mm
186007472
ACQUITY UPLC M-Class HSS T3
1.8 µm
75 µm x 150 mm
186007473
ACQUITY UPLC M-Class HSS T3
1.8 µm
75 µm x 250 mm
186007474
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
75 µm x 100 mm
186007475
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
75 µm x 150 mm
186007476
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
75 µm x 200 mm
186007477
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
75 µm x 250 mm
186007478
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
100 µm x 100 mm
186007479
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
150 µm x 100 mm
186007480
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
150 µm x 50 mm
186007513
ACQUITY UPLC M-Class CSH C
18
, 130Å
1.7 µm
150 µm x 150 mm
186007514
ACQUITY UPLC M-Class BEH C
18
, 130Å
1.7 µm
75 µm x 100 mm
186007481
ACQUITY UPLC M-Class BEH C
18
, 130Å
1.7 µm
75 µm x 150 mm
186007482
ACQUITY UPLC M-Class BEH C
18
, 130Å
1.7 µm
75 µm x 200 mm
186007483
ACQUITY UPLC M-Class BEH C
18
, 130Å
1.7 µm
75 µm x 250 mm
186007484
ACQUITY UPLC M-Class BEH C
18
, 130Å
1.7 µm
100 µm x 100 mm
186007485
ACQUITY UPLC M-Class BEH C
18
, 130Å
1.7 µm
150 µm x 100 mm
186007486
ACQUITY UPLC M-Class BEH C
18
, 300Å
1.7 µm
75 µm x 100 mm
186007487
ACQUITY UPLC M-Class BEH C
18
, 300Å
1.7 µm
100 µm x 100 mm
186007488
ACQUITY UPLC M-Class BEH C
18
, 300Å
1.7 µm
150 µm x 100 mm
186007489
ACQUITY UPLC M-Class BEH C
18
, 300Å
1.7 µm
75 µm x 150 mm
186007490
ACQUITY UPLC M-Class BEH C
18
, 300Å
1.7 µm
75 µm x 200 mm
186007491
ACQUITY UPLC M-Class BEH C
18
, 300Å
1.7 µm
75 µm x 250 mm
186007492
ACQUITY UPLC M-Class BEH C4, 300Å
1.7 µm
75 µm x 100 mm
186007493
ACQUITY UPLC M-Class BEH C4, 300Å
1.7 µm
100 µm x 100 mm
186007494
ACQUITY UPLC M-Class BEH C4, 300Å
1.7 µm
150 µm x 100 mm
186007495
ACQUITY UPLC M-Class Symmetry C
18
Trap, 100Å
5 µm
180 µm x 20 mm
186007496
1
ACQUITY UPLC M-Class Symmetry C
18
2D Trap, 100Å
5 µm
180 µm x 20 mm
186007497
2
ACQUITY UPLC M-Class Symmetry C
18
Trap, 100Å
5 µm
300 µm x 50 mm
186007498
1
ACQUITY UPLC M-Class Symmetry C
18
2D HCP Trap, 100Å
5 µm
300 µm x 25 mm
186007499
2
ACQUITY UPLC M-Class Symmetry C
18
V/V Trap, 100Å
5 µm
180 µm x 20 mm
186007500
1