Waters ACQUITY UPLC M-Class Columns - Manual (Page 4)

4
[ CARE AND USE MANUAL ]
Problem
 If a clog or a blockage is created after the ACQUITY UPLC
M-Class Column (e.g., at emitter) the pressure at the outlet
of the column may rise.
 The increase in pressure at the column outlet may create a
temporary leak between the column and the transfer tubing.
 The most common source of post column clogs is a blocked
nanospray emitter.
Note: Set high pressure limit on the ACQUITY UPLC M-Class
System to approximatively 1000 psi above maximum normal
operating pressure.
Diagnosis 1
The pressure traces in the console window show an increase in
pressure during the analytical gradient (red arrows) between two
injections (highlighted by the red squares).
Notice the pressure during the trapping portions of the analysis did
not increase between injections (red arrows).
Diagnosis 2
This shows the increase in system pressure must originate after the
trap column.
Representative diagram of ACQUITY UPLC M-Class System with attached trap and
ACQUITY UPLC M-Class Column.
ACQUITY UPLC M-Class Column Care & Use