Waters ACQUITY UPLC M-Class Columns - Manual (Page 3)

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[ CARE AND USE MANUAL ]
Carefully grab the teflon tubing with one hand and the BARE FUSED
SILICA capillary, not the PEEK tubing, with the other hand, to remove
the Teflon sleeve. Again, be careful NOT to apply unnecessary force
when removing the teflon tubing which could cause the fragile joint
between transfer tube and packed capillary to separate.
b) Connection
 Connecting and proper tightening of 75, 100, or 150 µm I.D.,
ACQUITY UPLC M-Class Columns to 15 K ACQUITY UPLC system.
 Flow mobile phase only in the direction indicated by the arrow
on the column label. Connect the column with the direction of
the flow arrow on the label pointing to the detector or Mass
Spectrometer.
1. For a column never installed on an ACQUITY UPLC
M-Class System.
 Turn nut until hand tight then an additional 1/2 turn
2. For a previously installed and removed column from
ACQUITY UPLC M-Class System.
 Hand tight plus 1/8 turn
“Hand Tight” is when the ferrule bottoms in the M-detail fitting.
The ferrule is pre-staked higher than necessary on the tube, so
the ferrule must be seated by hand (hand tight) or lightly with
a wrench, then an additional turn. Note: In virtually every case,
gentle wrenching is required to get to the “hand tight” state
before the final half turn. Taking “hand tight” or “finger tight” too
literally can result in under tightening/leaks.
Note: DO NOT over tighten, leaking may occur and/or the ferrule
may become stuck in the port.
IV. P REPARING AND CONNECTING
300 µM I.D. ACQUIT Y UPLC M-CLASS COLUMNS
Carefully remove column from box.
 Connect the column inlet to the injector utilizing a Valco style
compression screw.
 Connect the column outlet to the detector or Mass Spectrometer
using a Valco style compression screw.
V. CARE W HEN STOP PING FLOW
 Do not stop the flow to your ACQUITY UPLC M-Class Column
suddenly. It is critical that the flow be slowly lowered via
the ACQUITY UPLC M-Class System controller to prevent
column damage.
VI. HOW TO DIAGNOSE AND ADDRESS
ABNORMALLY HIGH BACK P RESSURE AND LEAKS
 The inlet or the head of a chromatography column experiences
the largest amount of pressure in a LC system. The
ACQUITY UPLC M-Class Column inlet is designed to
operate at pressures up to 15 K psi.
 The fluidic pressure drops across the column at the outlet of the
column should only experience atmospheric pressure. The outlet
connection which connects the column to the transfer capillary is
designed to handle a maximum pressure of 800 psi.
 With a higher than normal system pressure, a small leak might be
detected at the outlet of an ACQUITY UPLC M-Class Column.
Ferrule Nut
Wrong Correct
ACQUITY UPLC M-Class Column Care & Use