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Care and use manual – Waters ACQUITY UPC2 Trefoil Columns User Manual

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[ CARE AND USE MANUAL ]

ACQUITY UPC

2

Trefoil Columns Care & Use

Retest the column after using the cleaning procedure to
determine if the specific problem has been fixed. If so, continue
using the column, avoiding samples and solvents that may clog
the column inlet.

b. Effects of Additives

Use of co-solvent additives (e.g. ammonia, organic amines, formic
acid, trifluoroacetic acid) can be used successfully with all Trefoil
columns. Consider the volatility and detector compatibility when
choosing an appropriate additive. Additives tend to improve peak
shape and control the retention characteristics of analytes, but
can also impart different selectivities.

c. Storage

If additives were used, flush the column using a minimum of
5 column volumes 50% MeOH/CO

2

. Store the columns in

100% CO

2

.

VI. INT RODUCING ECORD INT ELLIGENT
CHIP T ECHNOLOGY

a. Introduction

The eCord Intelligent Chip will provide the history of a column’s
performance throughout its lifetime. The eCord is permanently
attached to the column to assure that the column’s performance
history is maintained in the event that the column is moved from
one instrument to another.

Figure 2. eCord Intelligent Chip.

At the time of manufacture, tracking and quality control
information will be downloaded to the eCord. Storing this
information on the chip will eliminate the need for a paper
Certificate of Analysis.

Once the user installs the column, the software will automatically
download key parameters into a column history file stored on
the chip. In this manual, we explain how the eCord will provide
a solution for easily tracking the history of the columns, reduce
the frustration of paperwork trails, and give customers the
reassurance that a well-performing column is installed onto
their instruments.

b. Installation

Install the column into the ACQUITY

®

Column Manager. Plug

the eCord into the side of the column heater. Once the eCord is
inserted into the column heater the identification and overall
column usage information will be available allowing the user
to access column information on their desktop.

Figure 3. Installing the eCord Intelligent Chip.

eCord Fob