Waters ACQUITY UPC2 Trefoil Columns - Manual (Page 2)

2
[ CARE AND USE MANUAL ]
ACQUITY UPC 2 Trefoil Columns Care & Use
The ACQUITY UPC 2 packing materials are designed for use
with the ACQUITY UPC 2 Systems and are manufactured in an
ISO-certified plant using ultra-pure reagents. Each batch of
ACQUITY UPC
2 material is tested and the results are held to
narrow specification ranges to assure excellent, reproducible
performance. Every column is individually tested and a
Performance Chromatogram and Certificate of Batch Analysis
is provided on the eCord™ Intelligent Chip.
ACQUITY UPC 2 Columns are designed and tested specifically for
use on ACQUITY UPC 2 Systems. ACQUITY UPC 2 Columns will
exhibit maximum chromatographic performance and benefit
ONLY when used on holistically-designed ACQUITY UPC
2 Systems
since these systems and columns were created and designed to
operate together. For these reasons, Waters cannot support the
use of ACQUITY UPC
2 Columns on any system other than an
ACQUITY UPC 2 System.
II. GET TING START ED
Each ACQUITY UPC 2 Column comes with a Certificate of Analysis
and a Performance Test Chromatogram. The Certificate of Analysis
is specific to each batch of packing material and includes the gel
batch number, physical characterization, analysis of unbonded
particle, analysis of bonded particles, and a SFC chromatographic
batch test. The Performance Test Chromatogram is specific to each
individual column and contains the following information: gel
batch number, column serial number, USP plate count, USP tailing
factor, capacity factor, and chromatographic conditions under
normal-phase LC conditions. These data should be stored
for future reference.
a. Safety Considerations
An SFC column, while in use, is under pressure with CO
2
and
possible modifiers as a supercritical fluid. A major safety concern
is frostbite caused by adiabatic cooling when CO
2
decompresses
from a fluid to a gas at atmospheric pressure. Pay attention to any
frosting on the column or system connections. This indicates a
leak, usually with temperatures far below 0 °C.
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
min
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
Chiral separations were all run using the 2-minute screening method.
O
O
O
H
N
O
OH
N
N
S
Flavanone
Oxprenolol
ACQUITY UPC 2 Trefoil AMY1
2.5 µm Columns
Amylose tris-(3,5-dimethylphenylcarbamate)
ACQUITY UPC 2 Trefoil CEL1
2.5 µm Columns
Cellulose tris-(3,5-dimethylphenylcarbamate)
ACQUITY UPC 2 Trefoil CEL2
2.5 µm Columns
Cellulose tris-(3-chloro-4-methylphenylcarbamate)
Tetramisole
ACQUITY UPC 2 Trefoil Columns for Chiral Separations
Trefoil Column: 2.1 x 50 mm
Co-solvent: 1:1 MeOH:IPA w/20 mM NH4OH
Gradient: 3–60% in 1.5 min, 60% for 0.5 min
Flow rate: 1.2 mL/min
Temperature: 40 °C
ABPR: 3,200 psi
UV Detection: 220 nm