Eppendorf R Series 48R CO2 Incubator - Manual (Page 61)

61
New Brunswick Scientific User’s Guide
filter that is already installed in the gas line. The gas flow rate is
approximately 20 liters/minute.
4. Install the oxygen cylinder in the same manner as outlined for the nitrogen
cylinder installation in Section 8.7.1, using the incubator’s O
2
inlet, of
course. For this application, you will need another two-stage pressure
regulator and another inline pressure regulator.
NOTE:
If the programmed O
2
level is close to the ambient oxygen, it may be
necessary to reduce the cylinder pressure below 1 bar to stop the oxygen
level from undershooting the programmed value.
Be sure to humidify the incubator and leave it overnight to stabilize before
proceeding further.
To automatically calibrate the sensor to atmospheric oxygen levels, select
OXYGEN SENSOR – REF TO ATMOSPHERE in the USER menu, and then follow
the onscreen instructions.
The oxygen reading is automatically adjusted to 19.7%, which is the true
reading taking into account the Relative Humidity in the chamber.
8.7.3 Enabling or Disabling O
2
Control
To enable oxygen control:
1. Select the
USER screen by pressing the USER function key.
2. Use the direction keys to select MANUAL DISABLE .
3. Press the ENTER function key, then use the direction keys to select ON for
Oxygen Control.
4. Press the ENTER function key again.
Program the required oxygen level in the
PROG screen, following the onscreen
instructions.
To disable oxygen control, follow the pattern used to enable it, but this time
select
USER , MANUAL DISABLE , ENTER , OFF and then ENTER again.