Eppendorf R Series 48R CO2 Incubator - Manual (Page 57)

57
New Brunswick Scientific User’s Guide
1. Remove the black plastic protective cover (making sure that the
hydrophobic filter cap is not removed with it) from the port inside the
chamber. Retain the cover for use when you clean the chamber.
2. Open the nitrogen gas supply from the tank and set the N
2
tank’s outlet
pressure gauge to 1.5 bar.
3. Set the nitrogen regulator to 1 bar (14.5 psi). The nitrogen will be fed into
the incubator through a HEPA filter that is already installed in the gas line.
The gas flow rate is approximately 20 liters/minute.
NOTE:
If the programmed O
2
level is close to the ambient oxygen, it may be
necessary to reduce the cylinder pressure below 1 bar to stop the oxygen
level from undershooting the programmed value.
NOTE:
When working at 0.1% O
2
, set the CO
2
pressure to 0.2 bar (2.9 psi).
Be sure to humidify the incubator and leave it overnight to stabilize before
proceeding further.
To automatically calibrate the sensor to atmospheric oxygen levels, select
OXYGEN SENSOR – REF TO ATMOSPHERE in the USER menu, and then follow
the onscreen instructions.
The oxygen reading is automatically adjusted to 19.7%, which is the true
reading taking into account the Relative Humidity in the chamber.
8.6.3 Enabling or Disabling O
2
Control
To enable oxygen control:
1. Select the
USER screen by pressing the USER function key.
2. Use the direction keys to select MANUAL DISABLE .
3. Press the ENTER function key, then use the direction keys to select ON for
Oxygen Control.
4. Press the ENTER function key again.
Program the required oxygen level in the PROG screen, following the onscreen
instructions.
To disable oxygen control, follow the pattern used to enable it, but this time
select
USER , MANUAL DISABLE , ENTER , OFF and then ENTER again.